THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling.
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Themis
Lys122-Lys237
Accession # Q8BGW0
Lys122-Lys237
Accession # Q8BGW0
Specificity
Detects mouse Themis in direct ELISAs and mouse and rat Themis in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Themis is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Themis Antibody [Biotin]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Themis
Long Name
Thymocyte-expressed Molecule Involved in Selection
Alternate Names
C6orf190, C6orf207, Gasp, SPOT, TSEPA
Gene Symbol
THEMIS
Additional Themis Products
Product Documents for Themis Antibody [Biotin]
Product Specific Notices for Themis Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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