Themis Antibody

Novus Biologicals | Catalog # NBP3-23438

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Themis Primary Antibodies »

Product Specifications

Immunogen

Themis (N-terminal region) antibody was generated from a peptide that included amino acids in the N-terminal region of human themis. This sequence has high homology to the conserved region in rat and mouse themis. [UniProt# Q8N1K5]

Specificity

The antibody detects a 72 kDa protein corresponding to the molecular mass of themis on SDS-PAGE immunoblots of mouse spleen and human K562 cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Themis Antibody

Themis Antibody

Western Blot: Themis Antibody [NBP3-23438] -

Western Blot: Themis Antibody [NBP3-23438] - Western blot of mouse spleen (lanes 1-4) and human K562 cells (lanes 5-8). The blots were probed with anti-themis (N-terminal region) rabbit polyclonal antibody at 1:2000 (lanes 1 & 5), 1:1000 (lanes 2 & 6), 1:500 (lanes 3 & 7), or 1:500 in the presence of themis blocking peptide (lanes 4 & 8).

Applications for Themis Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Themis

THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling.

Long Name

Thymocyte-expressed Molecule Involved in Selection

Alternate Names

C6orf190, C6orf207, Gasp, SPOT, TSEPA

Gene Symbol

THEMIS

Additional Themis Products

Product Documents for Themis Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Themis Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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