Thioredoxin-1 Antibody (116) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89458

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 116 expressed in HEK293

Format

Azide and BSA Free
View all Thioredoxin-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Thioredoxin-1 (Uniprot#: P10599; Met1-Val105).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Thioredoxin-1 Antibody (116) - Azide and BSA Free

Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458]

Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458]

Western Blot: Thioredoxin-1 Antibody (116) [NBP2-89458] - Lane A: Hela Whole Cell Lysate Lane B: THP-1 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:12 kDa Observed band size:14 kDa
Immunoprecipitation: Thioredoxin-1 Antibody (116) [NBP2-89458]

Immunoprecipitation: Thioredoxin-1 Antibody (116) [NBP2-89458]

Immunoprecipitation: Thioredoxin-1 Antibody (116) [NBP2-89458] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate2 uL anti-TXN rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-TXN rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 13 kDa

Applications for Thioredoxin-1 Antibody (116) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thioredoxin-1

Thioredoxins (Trxs) are a group of small ubiquitous proteins that are key regulators of cellular redox balance. The mammalian Trx family includes the secreted and cellular protein (Trx-1), the mitochondria-specific Trx-2, the Trx-like cytosolic protein p32TrxL, a truncated version of Trx-1 known as Trx80, and the thioredoxin-related protein, TRP-14. Trx-1 is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Trx-1 acts as a growth factor an, antioxidant, a cofactor that provides reducing equivalents, and a transcriptional regulator. Expression of Trx-1 is increased under various stress conditions such as hypoxia and viral and bacterial infections. It also serves as a biomarker for rheumatoid arthritis.

Alternate Names

Thioredoxin1, Trx1, TXN, TXN1

Gene Symbol

TXN

Additional Thioredoxin-1 Products

Product Documents for Thioredoxin-1 Antibody (116) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Thioredoxin-1 Antibody (116) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Thioredoxin-1 Antibody (116) - Azide and BSA Free

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FAQs

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail