Thioredoxin-1 Antibody (2A7) - Azide and BSA Free

Novus Biologicals | Catalog # H00007295-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A7

Format

Azide and BSA Free
View all Thioredoxin-1 Primary Antibodies »

Product Specifications

Immunogen

TXN (AAH03377, 1 a.a. ~ 105 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATINELV

Localization

Cytoplasmic and Secreted.

Specificity

TXN - thioredoxin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Thioredoxin-1 Antibody (2A7) - Azide and BSA Free

Western Blot: Thioredoxin-1 Antibody (2A7) [H00007295-M01]

Western Blot: Thioredoxin-1 Antibody (2A7) [H00007295-M01]

Western Blot: Thioredoxin Antibody (2A7) [H00007295-M01] - TXN monoclonal antibody (M01), clone 2A7 Analysis of TXN expression in HeLa.
Immunocytochemistry/ Immunofluorescence: Thioredoxin-1 Antibody (2A7) [H00007295-M01]

Immunocytochemistry/ Immunofluorescence: Thioredoxin-1 Antibody (2A7) [H00007295-M01]

Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of monoclonal antibody to TXN on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Thioredoxin-1 Antibody (2A7) [H00007295-M01]

Western Blot: Thioredoxin-1 Antibody (2A7) [H00007295-M01]

Western Blot: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of TXN expression in transfected 293T cell line by TXN monoclonal antibody (M01), clone 2A7.Lane 1: TXN transfected lysate(11.7 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: Thioredoxin-1 Antibody (2A7) [H00007295-M01]

Immunoprecipitation: Thioredoxin-1 Antibody (2A7) [H00007295-M01]

Immunoprecipitation: Thioredoxin Antibody (2A7) [H00007295-M01] - Analysis of TXN transfected lysate using anti-TXN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TXN MaxPab rabbit polyclonal antibody.
ELISA: Thioredoxin-1 Antibody (2A7) [H00007295-M01]

ELISA: Thioredoxin-1 Antibody (2A7) [H00007295-M01]

ELISA: Thioredoxin Antibody (2A7) [H00007295-M01] - Detection limit for recombinant GST tagged TXN is approximately 0.03ng/ml as a capture antibody.

Applications for Thioredoxin-1 Antibody (2A7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Thioredoxin-1

Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It is ubiquitous and found in many organisms from plants and bacteria to mammals. Multiple in vitro substrates for thioredoxin have been identified, including ribonuclease, choriogonadotropins, coagulation factors, glucocorticoid receptor, and insulin. Reduction of insulin is classically used as an activity test.[supplied by OMIM]

Alternate Names

Thioredoxin1, Trx1, TXN, TXN1

Entrez Gene IDs

7295 (Human)

Gene Symbol

TXN

OMIM

187700 (Human)

UniProt

AAH03377 (Human)

Additional Thioredoxin-1 Products

Product Documents for Thioredoxin-1 Antibody (2A7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Thioredoxin-1 Antibody (2A7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Thioredoxin-1 Antibody (2A7) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail