Thyroglobulin Antibody (CL0164) - BSA Free

Novus Biologicals | Catalog # NBP2-14784

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL0164

Format

BSA Free
View all Thyroglobulin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P01266, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Thyroglobulin Antibody (CL0164) - BSA Free

Western Blot: Thyroglobulin Antibody (CL0164) [NBP2-14784]

Western Blot: Thyroglobulin Antibody (CL0164) [NBP2-14784]

Western Blot: Thyroglobulin Antibody (CL0164) [NBP2-14784] - Lane 1: Marker [kDa], Lane 2: Human normal thyroid
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784]

Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784]

Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784] - Staining of human small intestine shows no positivity.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784]

Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784]

Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784] - Staining of human thyroid gland shows strong cytoplasmic positivity.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784]

Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784]

Immunohistochemistry-Paraffin: Thyroglobulin Antibody (CL0164) [NBP2-14784] - Staining of human thyroid cancer shows strong cytoplasmic positivity.
Thyroglobulin Antibody (CL0164) - BSA Free

Thyroglobulin Antibody (CL0164) [NBP2-14784] -

Analysis in human thyroid gland and liver tissues using NBP2-14784 antibody. Corresponding TG RNA-seq data are presented for the same tissues.
Thyroglobulin Antibody (CL0164) - BSA Free

Thyroglobulin Antibody (CL0164) [NBP2-14784] -

Staining of human liver shows no positivity in hepatocytes as expected.

Applications for Thyroglobulin Antibody (CL0164) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thyroglobulin

Thyroglobulin is synthesized by the follicular epithelial cells of the thyroid. Thyroglobulin is the glycoprotein precursor to the thyroid hormones. Its synthesis and metabolism have seemingly wasteful features. It has a molecular weight of 660,000, with 2 identical subunits of MW 300,000 and 10% sugars; yet its complete hydrolysis yields only 2 to 4 molecules of the iodothyroxines T4 and T3. Baas et al. (1986) showed that the TG gene encodes an 8.7-kb mRNA, covers at least 300 kb of genomic DNA and contains at least 37 exons separated by introns as large as 64 kb. A striking structural difference between the 5-prime and 3-prime parts of the gene suggested that it is composed of 2 evolutionarily different regions. The first 30 kb of DNA encodes 3 kb of the mRNA yielding an exon:intron ratio of 1:10, whereas the remaining 270 kb encodes 5.7 kb of the mRNA with a ratio of 1.47.

Alternate Names

AITD3, cog, TDH3, Tg, TGN

Gene Symbol

TG

Additional Thyroglobulin Products

Product Documents for Thyroglobulin Antibody (CL0164) - BSA Free

Certificate of Analysis

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Product Specific Notices for Thyroglobulin Antibody (CL0164) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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