TIMP-1 Antibody Pair [HRP]

Novus Biologicals | Catalog # NBP2-79290

Novus Biologicals
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Key Product Details

Assay Type

Sandwich ELISA

Assay Range

4.68-300 pg/ml (example only; lot dependent)

Sensitivity

4.68 pg/ml (example only; lot dependent)

Reactivity

Human
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Product Specifications

Description

Solid Phase sandwich ELISA for the quantitative determination of Human TIMP-1.

Sample Volume Required

100 ul

Conjugate

HRP

Scientific Data Images for TIMP-1 Antibody Pair [HRP]

Sandwich ELISA: TIMP-1 Antibody Pair [HRP] [NBP2-79290] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Kit Contents for TIMP-1 Antibody Pair [HRP]

  • Mouse Monoclonal Capture Antibody
  • Rabbit Polyclonal Detection Antibody (HRP-conjugated)
  • Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TIMP-1

Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Gene Symbol

TIMP1

Additional TIMP-1 Products

Product Documents for TIMP-1 Antibody Pair [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TIMP-1 Antibody Pair [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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