TIP30 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88170

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TIP30 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALSKLREDFRMQNKSVFILGASGETGRVLLKEILEQGLFSKVTLIGRRKLTFDEEAYKNVNQEVVDFEKLDDYASAFQGHDVGFC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TIP30 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TIP30 Antibody [NBP1-88170]

Immunocytochemistry/ Immunofluorescence: TIP30 Antibody [NBP1-88170]

Immunocytochemistry/Immunofluorescence: TIP30 Antibody [NBP1-88170] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170] - Staining of human skeletal muscle shows very weak positivity.
Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170] - Staining of human lymph node shows moderate cytoplasmic positivity.
Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170]

Immunohistochemistry-Paraffin: TIP30 Antibody [NBP1-88170] - Staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.
TIP30 Antibody - BSA Free Western Blot: TIP30 Antibody - BSA Free [NBP1-88170]

Western Blot: TIP30 Antibody - BSA Free [NBP1-88170]

Analysis in control (vector only transfected HEK293T lysate) and HTATIP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
TIP30 Antibody - BSA Free Western Blot: TIP30 Antibody - BSA Free [NBP1-88170]

Western Blot: TIP30 Antibody - BSA Free [NBP1-88170]

Analysis in human cell line A-549.

Applications for TIP30 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIP30

TIP30, also called CC3 or HTATIP2, is a putative metastasis suppressor that promotes apoptosis and inhibits angiogenesis. Although TIP30 has several characteristic features of a tumor suppressor in in vitro analyses, tumor development as a result of TIP30 inactivation has not been demonstrated in vivo, and abnormal expression of TIP30 in human cancer has not been reported.

Alternate Names

30 kDa HIV-1 TAT-interacting protein, CC3oxidoreductase HTATIP2, EC 1.1.1, EC 1.1.1.-, FLJ26963, HIV-1 Tat interactive protein 2, 30 kDa, HIV-1 Tat interactive protein 2, 30kDa, HIV-1 TAT-interactive protein 2, SDR44U1, Tat-interacting protein (30kD), TIP30short chain dehydrogenase/reductase family 44U, member 1

Gene Symbol

HTATIP2

Additional TIP30 Products

Product Documents for TIP30 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TIP30 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TIP30 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review TIP30 Antibody - BSA Free and earn rewards!

Have you used TIP30 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies