Human TMPRSS2 ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP2-89171

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

This Human TMPRSS2 ELISA Kit (Chemiluminescence)recognizes Human TMPRSS2 in samples. No significant cross-reactivity or interference between Human TMPRSS2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 391

Inter-Assay Precision (Precision between assays) CV% 389

Recovery for Human TMPRSS2 ELISA Kit (Chemiluminescence)

Recovery

87-112%

Linearity

Human TMPRSS2 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human TMPRSS2 ELISA Kit (Chemiluminescence)

Human TMPRSS2 ELISA Kit (Chemiluminescence)

ELISA: Human TMPRSS2 ELISA Kit (Chemiluminescence) [NBP2-89171] -

ELISA: Human TMPRSS2 ELISA Kit (Chemiluminescence) [NBP2-89171] - Standard Curve Reference

Kit Contents for Human TMPRSS2 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TMPRSS2

TMPRSS2, also called Epitheliasin in mice, is a 492 amino acid type II transmembrane serine protease located on human chromosome 21q22.3 that encodes at least 2 isoforms. This glycosylated serine protease (theoretical molecular weight 70kDa) is regulated by androgens and expressed on the plasma membrane of human bronchial epithelial cells, nasal goblet cells, small intestine epithelia, gastrointestinal tract, the stomach, kidneys and pancreas, with the greatest abundance found in the prostate gland. While the physiological function of TMPRSS2 remains unknown, the serine protease domain undergoes autocleavage and the 32 kDa domain is secreted enabling its interaction with the extracellular matrix. Fusions between TMPRSS2 and the ETS transcription factor genes ERG, ETV1, and ETV4 have been reported in prostate cancer with the TMPRSS2 ERG gene fusion resulting in ERG overexpression in 40-80% of these cases and often producing a more aggressive phenotype. Androgen signaling is disrupted in prostate cancers with the TMPRSS2 ERG fusion which contributes to the switch from androgen dependent to androgen independent prostate cancer (1,2).

TMPRSS2 has also been shown to play a critical role in the process of viral entry for coronaviruses and influenza viruses thru proteolytic activation of key viral glycoproteins. One pathway for SARS-CoV-2 infection, which causes the COVID-19 disease, involves binding of the SARS-CoV-2 Spike 'S' glycoprotein to the human ACE2 receptor and cleavage of S by TMPRSS2 at the cell surface to facilitate viral entry. TMPRSS2 mediates viral entry in a similar mechanism for other coronaviruses such as SARS-CoV and MERS. The broad-spectrum serine protease inhibitor, Camostat, is a TMPRSS2 inhibitor demonstrated to protect mice with lethal SARS-CoV infections (3).

References

1. Clark, J., Cooper, C. (2009) ETS gene fusions in prostate cancer. Nat Rev Urol 6, 429-439. PMID: 19657377

2. Duffy, MJ. (2014) Chapter One - PSA in Screening for Prostate Cancer: More Good than Harm or More Harm than Good? Adv Clin Chem. 66:1-23. PMID: 25344984

3. Shen LW, Mao HJ, Wu YL, Tanaka Y, Zhang W. (2017) TMPRSS2: A potential target for treatment of influenza virus and coronavirus infections. Biochimie. 142:1-10

Long Name

Transmembrane protease, serine 2

Alternate Names

Epitheliasin, PP9284, PRSS10

Gene Symbol

TMPRSS2

Additional TMPRSS2 Products

Product Documents for Human TMPRSS2 ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human TMPRSS2 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Serine Proteases and Regulators

Citations for Human TMPRSS2 ELISA Kit (Chemiluminescence)

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FAQs for Human TMPRSS2 ELISA Kit (Chemiluminescence)

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    • A: The recombinant extracellular domain of human TMPRSS2 (106-492aa) was applied as the antigen for the antibodies used in this kit. And the standard is also recombinant human TMPRSS2 106-492aa protein. Therefore, this kit is specific to the extracellular domain.
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