TMUB2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-48419

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all TMUB2 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant human TMUB2 (RefSeq#: NP_803190.2; Tyr37-Ser245).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.

Scientific Data Images for TMUB2 Antibody - Azide and BSA Free

TMUB2 Antibody - Azide and BSA Free TMUB2 Antibody - Azide and BSA Free

Western Blot: TMUB2 Antibody - Azide and BSA Free [NBP3-48419] -

Western Blot: TMUB2 Antibody - Azide and BSA Free [NBP3-48419] - Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane using NBP3-48419 at 1:500 dilution. Secondary: HRP-conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 37 kDa
TMUB2 Antibody - Azide and BSA Free TMUB2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TMUB2 Antibody - Azide and BSA Free [NBP3-48419] -

Immunocytochemistry/ Immunofluorescence: TMUB2 Antibody - Azide and BSA Free [NBP3-48419] - Immunofluorescence staining of TMUB2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-48419 (dilution ratio 1:100) at 4C overnight. Then cells were stained with Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.
TMUB2 Antibody - Azide and BSA Free TMUB2 Antibody - Azide and BSA Free

Immunoprecipitation: TMUB2 Antibody - Azide and BSA Free [NBP3-48419] -

Immunoprecipitation: TMUB2 Antibody - Azide and BSA Free [NBP3-48419] - Lane A: 0.5 mg HeLa Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Blotted with 4 uL anti-TMUB2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Immunoprecipitated using NBP3-48419 at 1:100 dilution and HRP-conjugated secondary antibody at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size :38 kDa

Applications for TMUB2 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1-5 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: TMUB2

Alternate Names

MGC3123, transmembrane and ubiquitin-like domain containing 2, transmembrane and ubiquitin-like domain-containing protein 2

Gene Symbol

TMUB2

Additional TMUB2 Products

Product Documents for TMUB2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TMUB2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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