TPR Antibody

Novus Biologicals | Catalog # NB100-2866

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TPR Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2275 and 2325 of human Translocated Promoter Region Protein using the numbering given in entry NP_003283.1 (GeneID 7175).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

267 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TPR Antibody

Western Blot: TPR Antibody [NB100-2866]

Western Blot: TPR Antibody [NB100-2866]

Western Blot: TPR Antibody [NB100-2866] - Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TPR antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.
Immunocytochemistry/ Immunofluorescence: TPR Antibody [NB100-2866]

Immunocytochemistry/ Immunofluorescence: TPR Antibody [NB100-2866]

Immunocytochemistry/Immunofluorescence: TPR Antibody [NB100-2866] - TPR and Mad1 interaction at the Nuclear Pores (green dots). DNA counterstained with DAPI (blue). Image submitted by a verified customer review.
Western Blot: TPR Antibody [NB100-2866]

Western Blot: TPR Antibody [NB100-2866]

Western Blot: TPR Antibody [NB100-2866] - Detection of Human TPR on HeLa whole cell lysate using NB100-2866. TPR was also immunoprecipitated by rabbit anti-TPR antibodies NB100-2864, NB100-2865 and NB100-2867.
Knockdown Validated: TPR Antibody [NB100-2866]

Western Blot: TPR Antibody [NB100-2866]

Western Blot: TPR Antibody [NB100-2866] - Lane 1 contians wildtype MDA-MB-231 cell lysate nuclear extract. Lanes 2, 3 and 4 contain the TPR knocked down MDA-MB-231 cell lysate nuclear extract. The lowered intensity of the bands in knocked down cell lines compared to wildtype supports antibody specificity to intended target protein. Image was submitted by customer review.

Applications for TPR Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 24344181) and customer review. TPR antibody validated for KD from a verified customer review.

Reviewed Applications

Read 2 reviews rated 5 using NB100-2866 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TPR

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias.

Alternate Names

nuclear pore complex-associated protein TPR, nucleoprotein TPR, translocated promoter region (to activated MET oncogene), tumor potentiating region

Entrez Gene IDs

7175 (Human)

Gene Symbol

TPR

UniProt

NP_003283.1 (Human)

Additional TPR Products

Product Documents for TPR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TPR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TPR Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • TPR Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 Cell Lysate nuclear Extract and MDA-MB-231 Cell Lysate nuclear extract
    Species: Human
    Verified Customer | Posted 01/24/2018
    Western Blot of MDA-MB-231 nuclear extracts. Samples are control and three different TPR knockdowns.
    Dilution 1:5000
    TPR Antibody NB100-2866
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested:
    Species: Human
    Verified Customer | Posted 08/27/2014
    Proximity Ligation Assay - TPR and Mad1 interaction at the Nuclear Pores (green dots). DNA counterstained with DAPI (blue).
    TPR Antibody NB100-2866

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Protocols

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FAQs

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