Transferrin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-97472

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Guinea Pig

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Transferrin Primary Antibodies »

Product Specifications

Immunogen

Human Transferrin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Transferrin Antibody - BSA Free

Western Blot: Transferrin Antibody [NBP1-97472]

Western Blot: Transferrin Antibody [NBP1-97472]

Transferrin-Antibody-Western-Blot-NBP1-97472-img0002.jpg
Western Blot: Transferrin Antibody [NBP1-97472]

Western Blot: Transferrin Antibody [NBP1-97472]

Western Blot: Transferrin Antibody [NBP1-97472] - Analysis of rat apotransferrin protein probed with transferrin pAb.
Transferrin Antibody

Western Blot: Transferrin Antibody [NBP1-97472] -

Western Blot: Transferrin Antibody [NBP1-97472] - Transfer of hippocampal/hypothalamic PTMS to other brain regions & the blood.(A, B) 3-mo male C57BL/6 mice were injected with a dose of synapsin promoter-driven PTMS-HA lentivirus in one side of the posterior hypothalamus (A) or both sides of the hippocampus targeting CA1 & dentate gyrus (B). At 2 wk post injection, brain sections were immunostained for HA in the injected regions versus other brain areas. Small arrows pointed to some HA positive cells in transfer brain regions. Scale bar, 100 μm. Additional information of control lentivirus injections are presented in Fig S6. (C, D) 3-mo male C57BL/6 mice received bilateral injections of neuron-specific lentivirus of synapsin promoter-driven PTMS-HA (labelled as “PTMS-HA”) versus Control-HA (labelled as “Con-HA”) in the hippocampus (targeting CA1 & dentate gyrus) & posterior hypothalamus, & at 2-wk post injection, secretion of PTMS-HA to the CSF (C) & the serum (D) was assessed via IP-Western blotting. (C, D) Transferrin blot (C) or Ponceau S staining (D) was used as loading control. **P < 0.01 (two-tailed unpaired t test), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Transferrin Antibody

Western Blot: Transferrin Antibody [NBP1-97472] -

Western Blot: Transferrin Antibody [NBP1-97472] - PTMS secretion declines during aging.(A, B) PTMS levels in the CSF of male C57BL/6 mice at young (∼4 mo) or old (∼24 mo) age were measured by Western blot (A) which were quantified in panel (B). Transferrin was used to as a loading control. (C) Young (3-mo) or middle aged (18-mo) male C57BL/6 mice received unilateral injection of the same dose of Sox2 promoter-driven PTMS-HA lentivirus in the mediobasal hypothalamus. White arrows point to the site of injections. At 1 wk postinjection, hypothalamic sections were generated for HA immunostaining to show the levels of both primary expression of PTMS-HA in Sox2-positive cells & secondary transfer of PTMS-HA into Sox2-negative cells. Sox2 co-immunostaining was performed to reveal Sox2-positive cells in this region. Lower panels in show high-magnification views of outlined areas. Scale bar, 100 μm. 3V: hypothalamic third ventricle. *P < 0.05, two-tailed unpaired t test (B), n = 3 mice per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Transferrin Antibody

Western Blot: Transferrin Antibody [NBP1-97472] -

Western Blot: Transferrin Antibody [NBP1-97472] - Contribution of the hypothalamus to PTMS in the CSF.(A) PTMS levels in blood serum versus CSF were detected by immunoprecipitation (IP)-Western blot (left panel), & the band intensities (au, arbitrary unit) were quantified (right panel). Matched IgG was used as a negative control of IP procedure, & Commassie staining was used as a loading control. (B, C) Injection of ptms-shRNA (sh-ptms) or scramble control shRNA (sh-control) lentiviruses into hypothalamic third ventricle of 3-mo male C57BL/6 mice (B), & at 1 mo postinjection, PTMS levels in CSF were measured by Western blot (left panel, transferrin as a loading control) & quantified (right panel). Scale bar, 100 μm. (A, C) *P < 0.05 (two-tailed unpaired t test), n = 4 mice (A) or 3 mice (C) per group; values represent the mean ± SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33087487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Transferrin Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Transferrin

Transferrin is a serum glycoprotein involved in iron transport. Transferrin exists in two forms, the iron-free Apotransferrin, which binds two Fe3+ ions to generate Ferrotransferrin. Apotransferrin remains tightly bound to its receptor until the complex is recycled to the cell surface, where Apotransferrin is released into the extracellular space to recruit more Fe3+ ions.

Alternate Names

Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF

Entrez Gene IDs

7018 (Human); 22041 (Mouse); 24825 (Rat)

Gene Symbol

TF

UniProt

P02787 (Human)

Additional Transferrin Products

Product Documents for Transferrin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Transferrin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Transferrin Antibody - BSA Free

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