TRAP alpha Antibody (OTI4C7)

Novus Biologicals | Catalog # NBP2-01026

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4C7
View all TRAP alpha Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SSR1(NP_003135) produced in HEK293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRAP alpha Antibody (OTI4C7)

Western Blot: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Western Blot: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP alpha.
Immunocytochemistry/ Immunofluorescence: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunocytochemistry/ Immunofluorescence: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP alpha.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human thyroid tissue using anti-TRAP alpha mouse monoclonal antibody.
Flow Cytometry: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Flow Cytometry: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Flow Cytometry: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRAP alpha antibody, and then analyzed by flow cytometry.
Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP alpha monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunocytochemistry/ Immunofluorescence: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody (4C7) [NBP2-01026] - Immunofluorescent staining of HeLa cells using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human endometrium tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (OTI4C7) [NBP2-01026]

Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human pancreas tissue using anti-TRAP alpha mouse monoclonal antibody.

Applications for TRAP alpha Antibody (OTI4C7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Reviewed Applications

Read 1 review rated 5 using NBP2-01026 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.95 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRAP alpha

The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Alternate Names

DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, signal sequence receptor, alpha, SSR alpha subunit, SSR-alpha, translocon-associated protein alpha subunit, translocon-associated protein subunit alpha, TRAP alpha, TRAP-alpha, TRAPASignal sequence receptor subunit alpha

Entrez Gene IDs

6745 (Human)

Gene Symbol

SSR1

Additional TRAP alpha Products

Product Documents for TRAP alpha Antibody (OTI4C7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TRAP alpha Antibody (OTI4C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRAP alpha Antibody (OTI4C7)

Customer Reviews for TRAP alpha Antibody (OTI4C7) (1)

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Customer Images

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  • Name: Marie-estelle LOSFELD
    Application: Western Blot
    Sample Tested: human dermal fibroblasts
    Species: Human
    Verified Customer | Posted 02/27/2014
    SSR1 detection in fibroblasts
    TRAP alpha Antibody (OTI4C7) NBP2-01026

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