TRIS base

Tocris Bioscience | Catalog # 3163

Commonly used laboratory reagent; used to make TBS, TAE and TBE buffers
Tocris Bioscience

Key Product Details

Description

Commonly used laboratory reagent; used to make TBS, TAE and TBE buffers
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Product Description

TRIS base is a commonly used laboratory reagent. Highly water soluble. Working pH range 7 - 9. Used in buffer solutions such as Tris-buffered saline (TBS), Tris-acetate-EDTA (TAE) in combination with acetic acid and EDTA (Cat. No. 2811) and Tris-borate-EDTA in combination with boric acid and EDTA.

Product Specifications for TRIS base

Molecular Weight

121.14

Formula

C4H11NO3

Storage

Store at RT

Purity

≥99.5%

Chemical Name

2-Amino-2-(hydroxymethyl)-1,3-propanediol

CAS Number

77-86-1

PubChem ID

6503

InChI Key

LENZDBCJOHFCAS-UHFFFAOYSA-N

SMILES

OCC(CO)(N)CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 363.42 3000

Preparing Stock Solutions for TRIS base

The following data is based on the product molecular weight 121.14.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
30 mM 0.28 mL 1.38 mL 2.75 mL
150 mM 0.06 mL 0.28 mL 0.55 mL
300 mM 0.03 mL 0.14 mL 0.28 mL
1500 mM 0.01 mL 0.03 mL 0.06 mL

Calculators

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 6 publications citing the usage of this product.

There are currently no references for this product.

Product Documents for TRIS base

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TRIS base

For research use only

Customer Reviews for TRIS base (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
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  • test
    Name: Chi Ngo
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: various cell lines, including AGS, HepG2.
    Verified Customer | Posted 09/06/2025
    I used Tris base to prepare the Tris buffer for various uses in molecular biology assays.
  • Western blot
    Name: Anonymous
    Species: Lysates from different species
    Assay Type: In Vitro
    Verified Customer | Posted 07/28/2018
    We have used this product to make western blot gels of different percentages of acrylamide
  • Works as expected
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 01/04/2018
    I used this for making TBS buffers for western blots. All seemed to work fine.

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