TROY/TNFRSF19 Overexpression Lysate

Name: TROY/TNFRSF19 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-17153
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBL1-17153

Submit a review

Product Documents

100% Guarantee

Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for TROY/TNFRSF19 Overexpression Lysate

TROY/TNFRSF19 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC214944

Accession#: NM_018647

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for TROY/TNFRSF19 Overexpression Lysate

Western Blot: TROY/TNFRSF19 Overexpression Lysate [NBL1-17153]

Western Blot: TROY Overexpression Lysate (Adult Normal) [NBL1-17153] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TROY.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: TROY/TNFRSF19

TROY is a member of the TNF receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate the JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Long Name

Tumor Necrosis Factor Receptor Superfamily, Member 19

Alternate Names

TAJ, TNFRSF19

Gene Symbol

TNFRSF19

Additional TROY/TNFRSF19 Products

Product Documents for TROY/TNFRSF19 Overexpression Lysate

Download Product Datasheets

Download SDS

Product Specific Notices for TROY/TNFRSF19 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Related Research Areas

Glial Lineage Markers

TNF Superfamily

Customer Reviews for TROY/TNFRSF19 Overexpression Lysate

There are currently no reviews for this product. Be the first to review TROY/TNFRSF19 Overexpression Lysate and earn rewards!

Have you used TROY/TNFRSF19 Overexpression Lysate?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image