TRPM7 Antibody (JE38-36)

Western Blot: TRPM7 Antibody (JE38-36) [NBP3-33061]

Western Blot: TRPM7 Antibody (JE38-36) [NBP3-33061] - Western blot analysis of TRPM7 on different lysates with Rabbit anti-TRPM7 antibody (NBP3-33061) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HCT 116 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 213 kDa
Observed band size: 250 kDa

Exposure time: 1 minute 55 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33061) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: TRPM7 Antibody (JE38-36) [NBP3-33061]

Flow Cytometry: TRPM7 Antibody (JE38-36) [NBP3-33061] - Flow cytometric analysis of HeLa cells labeling TRPM7.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33061, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).