TSC22/TSC22D1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-98963

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all TSC22 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TSC22/TSC22D1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TSC22/TSC22D1 Antibody - Azide and BSA Free

Western Blot: TSC22/TSC22D1 Antibody [NBP2-98963]

Western Blot: TSC22/TSC22D1 Antibody [NBP2-98963]

Western Blot: TSC22/TSC22D1 Antibody [NBP2-98963] - Anti-TSC22/TSC22D1 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 110 kDa. Observed band size: 120 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: TSC22/TSC22D1 Antibody [NBP2-98963]

Immunoprecipitation: TSC22/TSC22D1 Antibody [NBP2-98963]

Immunoprecipitation: TSC22/TSC22D1 Antibody [NBP2-98963] - TSC22/TSC22D1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate Lane B: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-TSC22/TSC22D1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-TSC22/TSC22D1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 110 kDa. Observed band size: 110 kDa
TSC22/TSC22D1 Antibody - Azide and BSA Free

Western Blot: TSC22/TSC22D1 Antibody - Azide and BSA Free [NBP2-98963] -

Functional impact of TSC22D1 knockdown in gastric cancer cells. qRT-PCR (A) and Western blot (B) analyses were performed to assess TSC22D1 expression levels following siRNA-mediated TSC22D1 knockdown in AGS and AGS-EBV cells. MTT assay (C) and colony formation assay (D) were conducted to assess the impact of TSC22D1 knockdown on cell proliferation and oncogenicity. Both experiments were independently performed three times, and representative results are shown in (C,D). (E) Wound healing assay was performed to assess the effect of TSC22D1 knockdown on cell migration in AGS and AGS-EBV cells. (F) Flow cytometry analysis using Annexin V/7-AAD staining was conducted to evaluate apoptosis following TSC22D1 knockdown in AGS and AGS-EBV cells. p-values: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41472023), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TSC22/TSC22D1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSC22

Transforming growth factor-beta-stimulated clone-22 (TSC-22) acts as a transcriptionalregulator to modulate cell growth and differentiation and cell death. TSC-22 contains a leucine zipper domain as well as a nuclear export signal, resulting in cytoplasmic localization in living cells. However, concomitant with the induction of apoptosis, TSC-22 translocates from the cytoplasm to the nucleus and shows transcriptional regulatory activity. TSC-22 acts as a major downstream component in the TGF-beta pathway, and also the PPARgamma signalling pathway. The association of these two pathways with tumor suppression, and the significant downregulation of TSC-22 mRNA in various cancer types, such as brain and salivary gland tumors, imply an antiproliferative role for TSC-22.

Long Name

TSC22 Domain Family, Member 1

Alternate Names

Egr5, TGFB1I4, TSC22D1

Gene Symbol

TSC22D1

Additional TSC22 Products

Product Documents for TSC22/TSC22D1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TSC22/TSC22D1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TSC22/TSC22D1 Antibody - Azide and BSA Free

Customer Reviews for TSC22/TSC22D1 Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review TSC22/TSC22D1 Antibody - Azide and BSA Free and earn rewards!

Have you used TSC22/TSC22D1 Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies