Human TSH beta ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60150

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

0.15 uIU/mL

Assay Range

0.3 uIU/mL - 12 uIU/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human TSH. No significant cross-reactivity or interference between human TSH and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 15

Inter-Assay Precision (Precision between assays) %CV 15

Scientific Data Images for Human TSH beta ELISA Kit (Colorimetric)

Human TSH beta ELISA Kit (Colorimetric)

Human TSH beta ELISA Kit (Colorimetric) [NBP2-60150] -

Human TSH beta ELISA Kit (Colorimetric) [NBP2-60150] - Standard Curve Reference

Kit Contents for Human TSH beta ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TSH beta

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for TSH. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Thyroid-stimulating Hormone Subunit beta

Alternate Names

Thyrotropin beta, TSHB

Gene Symbol

TSHB

Additional TSH beta Products

Product Documents for Human TSH beta ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human TSH beta ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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