Recombinant Human TSLP His Protein

Novus Biologicals | Catalog # NBP2-51957

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
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Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 29-159 of Human TSLP

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSYDFTNCD FEKIKAAYLS TISKDLITYM SGTKSTEFNN TVSCSNRPHC LTEIQSLTFN PTAGCASLAK EMFAMKTKAA LAIWCPGYSE TQINATQAMK KRRKRKVTTN KCLEQVSQLQ GLWRRFNRPL LKQQ

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

17.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TSLP His Protein

SDS-PAGE: Recombinant Human TSLP His Protein [NBP2-51957]

SDS-PAGE: Recombinant Human TSLP His Protein [NBP2-51957]

SDS-Page: Recombinant Human TSLP Protein [NBP2-51957] - 15% SDS Page (3 ug)

Formulation, Preparation, and Storage

NBP2-51957
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSLP

Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Gene Symbol

TSLP

Additional TSLP Products

Product Documents for Recombinant Human TSLP His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human TSLP His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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