Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-06808

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

4.69 pg/mL

Assay Range

7.81 - 500 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human TWEAK/TNFSF12 in samples. No significant cross-reactivity or interference between Human TWEAK/TNFSF12 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.07

Inter-Assay Precision (Precision between assays) 5.22

Recovery for Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

Recovery

87 - 110 %

Linearity

Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

Scientific Data Images for Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

ELISA: Human TWEAK/TNFSF12 ELISA Kit (Colorimetric) [NBP3-06808] -

ELISA: Human TWEAK/TNFSF12 ELISA Kit (Colorimetric) [NBP3-06808] - Standard Curve Reference

Kit Contents for Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TWEAK/TNFSF12

CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia. ITEM-4 antibody reacts with both mouse and human Fn14. It is useful for flow cytometric analysis.

Long Name

TNF-like and Weak Inducer of Apoptosis

Alternate Names

TNFSF12

Gene Symbol

TNFSF12

Additional TWEAK/TNFSF12 Products

Product Documents for Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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