Recombinant Human U2AF35 His Protein

Name: Recombinant Human U2AF35 His Protein
Brand: Novus Biologicals
SKU: NBP2-22915
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-22915

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all U2AF35 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-240 of Human U2AF35

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTIL IQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKF RREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSR DRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

30.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human U2AF35 His Protein

SDS-PAGE: Recombinant Human U2AF35 His Protein [NBP2-22915]

SDS-Page: Recombinant Human U2AF35 Protein [NBP2-22915] - 15% SDS-PAGE (3 ug)

Formulation, Preparation, and Storage

NBP2-22915

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: U2AF35

U2AF1 belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This protein is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Recombinant human U2AF1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

FP793, human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712, RN, RNU2AF1,35-KD subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 small nuclear RNA auxillary factor 1, U2 snRNP auxiliary factor small subunit

Gene Symbol

U2AF1

Additional U2AF35 Products

Product Documents for Recombinant Human U2AF35 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human U2AF35 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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