UBE2O Antibody

Novus Biologicals | Catalog # NBP1-03336

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all UBE2O Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 725 and 775 of human ubiquitin-conjugating enzyme E2O using the numbering given in entry NP_071349.2 (GeneID 63893).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE2O Antibody

Western Blot: UBE2O Antibody [NBP1-03336]

Western Blot: UBE2O Antibody [NBP1-03336]

Western Blot: UBE2O Antibody [NBP1-03336] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiUBE2O antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: UBE2O Antibody [NBP1-03336]

Western Blot: UBE2O Antibody [NBP1-03336]

Western Blot: UBE2O Antibody [NBP1-03336] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE2O antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation: UBE2O Antibody [NBP1-03336]

Immunoprecipitation: UBE2O Antibody [NBP1-03336]

Immunoprecipitation: UBE2O Antibody [NBP1-03336] - Detection of human UBE2O by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBE2O antibody A301-873A (lot A301-873A-3) used for IP at 3 ug per reaction. UBE2O was also immunoprecipitated by rabbit anti-UBE2O antibody BL7369. For blotting immunoprecipitated UBE2O, A301-873A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for UBE2O Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UBE2O

UBE2O catalyzes the covalent attachment of ubiquitin to other proteins

Alternate Names

E2-230K, EC 6.3.2.19, FLJ12878, KIAA1734, Ubiquitin carrier protein O, ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-conjugating enzyme E2-230K, ubiquitin-conjugating enzyme E2O, Ubiquitin-protein ligase O

Entrez Gene IDs

63893 (Human)

Gene Symbol

UBE2O

UniProt

Q9C0C9 (Human)

Additional UBE2O Products

Product Documents for UBE2O Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UBE2O Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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