Human Ubiquitin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-49980

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.5 pg/mL

Assay Range

62.5 - 4000 pg/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human Ubiquitiniquitin in samples. No significant cross-reactivity or interference between Human Ub and analogues was observed

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% 10

Inter-Assay Precision (Precision between assays) CV% 10

Recovery for Human Ubiquitin ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Human Ubiquitin ELISA Kit (Colorimetric)

Scientific Data Images for Human Ubiquitin ELISA Kit (Colorimetric)

Human Ubiquitin ELISA Kit (Colorimetric)

ELISA: Human Ubiquitin ELISA Kit (Colorimetric) [NBP3-49980]

Standard Curve Reference.

Kit Contents for Human Ubiquitin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Ubiquitin

Ubiquitin is a highly conserved protein of approximately 8.5kDa molecular weight, which has a normal role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C-terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

Alternate Names

UBB

Gene Symbol

RPS27A

Additional Ubiquitin Products

Product Documents for Human Ubiquitin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Ubiquitin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs

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