UCH-L3 Antibody (153) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90679

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 153 expressed in HEK293

Format

Azide and BSA Free
View all UCH-L3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse UCH-L3 (Uniprot#: Q9JKB1; Glu2-Ala230).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UCH-L3 Antibody (153) - Azide and BSA Free

Western Blot: UCH-L3 Antibody (153) [NBP2-90679]

Western Blot: UCH-L3 Antibody (153) [NBP2-90679]

Western Blot: UCH-L3 Antibody (153) [NBP2-90679] - Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa. Observed band size:26 kDa. (We are unsure as to the identity of these extra bands.)
Immunoprecipitation: UCH-L3 Antibody (153) [NBP2-90679]

Immunoprecipitation: UCH-L3 Antibody (153) [NBP2-90679]

Immunoprecipitation: UCH-L3 Antibody (153) [NBP2-90679] - Mouse UCHL3 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. Lane B:0.5 mg 293T Whole Cell Lysate. 2 uL anti-Mouse UCHL3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse UCHL3 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody:

Applications for UCH-L3 Antibody (153) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

0.5-2 ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L3

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8

Long Name

Ubiquitin C-terminal Hydrolase L3

Alternate Names

Ubiquitin Thioesterase L3, UCHL3

Gene Symbol

UCHL3

Additional UCH-L3 Products

Product Documents for UCH-L3 Antibody (153) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UCH-L3 Antibody (153) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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