Q: Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.

A: Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.

Human UCP2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82387

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Product Documents

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human UCP2 in samples. No Significant cross-reactivity or interference between Human UCP2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.45

Inter-Assay Precision (Precision between assays) CV% 5.36

Recovery for Human UCP2 ELISA Kit (Colorimetric)

Recovery

94-107%

Linearity

Scientific Data Images for Human UCP2 ELISA Kit (Colorimetric)

ELISA: Human UCP2 ELISA Kit (Colorimetric) [NBP2-82387] -

ELISA: Human UCP2 ELISA Kit (Colorimetric) [NBP2-82387] - Standard Curve Reference

Kit Contents for Human UCP2 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: UCP2

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'.

Long Name

Uncoupling Protein 2

Alternate Names

SLC25A8, UCPH

Gene Symbol

UCP2

Additional UCP2 Products

Product Documents for Human UCP2 ELISA Kit (Colorimetric)

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Download Manual - Human UCP2 ELISA Kit (Colorimetric)

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Product Specific Notices for Human UCP2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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