UFM1 Activating Enzyme/UBA5 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-30632
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Between 325 and 375
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UFM1 Activating Enzyme/UBA5 Antibody - BSA Free
Immunoprecipitation:UFM1 Activating Enzyme/UBA5 AntibodyNBP3-30632] -
Immunoprecipitation:UFM1 Activating Enzyme/UBA5 AntibodyNBP3-30632] -Detection of human UBA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBA5 antibody NBP3-30632 (lot NBP3-30632-2) used for IP at 6 µg per reaction. UBA5 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30632-1). For blotting immunoprecipitated UBA5, NBP3-30632 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for UFM1 Activating Enzyme/UBA5 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate buffer, pH 7 to 8
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: UFM1 Activating Enzyme/UBA5
Alternate Names
THIFP1, UBA5, UBE1DC1
Gene Symbol
UBA5
Additional UFM1 Activating Enzyme/UBA5 Products
Product Documents for UFM1 Activating Enzyme/UBA5 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for UFM1 Activating Enzyme/UBA5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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