Recombinant E. coli UNG His Protein

Name: Recombinant E. coli UNG His Protein
Brand: Novus Biologicals
SKU: NBP2-22637
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-22637

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all UNG Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-229 of E.coli UNG

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli UNG His Protein

SDS-PAGE: Recombinant E. coli UNG His Protein [NBP2-22637]

SDS-Page: uracil-DNA glycosylase Protein [NBP2-22637]

Formulation, Preparation, and Storage

NBP2-22637

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UNG

Ung, also known as Uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Recombinant E.coli ung protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Uracil N-Glycosylase

Alternate Names

DGU, UDG

Gene Symbol

UNG

Additional UNG Products

Product Documents for Recombinant E. coli UNG His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant E. coli UNG His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Base Excision Repair

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