Universal Chemiluminescent PARP Assay - Histone Coated Wells

For the in vitro screening of candidate PARP-1 inhibitors
Catalog # Availability Size / Price Qty
4676-096-K
Product Details
FAQs
Reviews

Universal Chemiluminescent PARP Assay - Histone Coated Wells Summary

Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits

• Chemiluminescent readout
• 96 strip-well format
• Sensitive – detects 10 mU PARP /well
• Assay Time ~3 hrs

Why Use the Universal Chemiluminescent PARP Assay Kit?

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.

Product Specifications

For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents

• 3-Aminobenzamide
• Human PARP 1 Enzyme, HSA
• 20X PARP Buffer
• 10X PARP Cocktail
• 10X Activated DNA
• 10X Strep-Diluent
• PeroxyGlow™ A
• PeroxyGlow™ B
• Histone-Coated White Strip Well Plate
• Strep-HRP

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

FAQs

No product specific FAQs exist for this product, however you may

View all FAQs

Reviews for Universal Chemiluminescent PARP Assay - Histone Coated Wells

There are currently no reviews for this product. Be the first to review Universal Chemiluminescent PARP Assay - Histone Coated Wells and earn rewards!

Have you used Universal Chemiluminescent PARP Assay - Histone Coated Wells?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review