Universal Chemiluminescent PARP Assay - Histone Coated Wells
Universal Chemiluminescent PARP Assay - Histone Coated Wells Summary
• Chemiluminescent readout
• 96 strip-well format
• Sensitive – detects 10 mU PARP /well
• Assay Time ~3 hrs
Why Use the Universal Chemiluminescent PARP Assay Kit?
This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.
For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.
• Human PARP 1 Enzyme, HSA
• 20X PARP Buffer
• 10X PARP Cocktail
• 10X Activated DNA
• 10X Strep-Diluent
• PeroxyGlow™ A
• PeroxyGlow™ B
• Histone-Coated White Strip Well Plate
For research use only. Not for diagnositic use.
Citations for Universal Chemiluminescent PARP Assay - Histone Coated Wells
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Uncoupling of PARP1 trapping and inhibition using selective PARP1 degradation
Authors: S Wang, L Han, J Han, P Li, Q Ding, QJ Zhang, ZP Liu, C Chen, Y Yu
Nat. Chem. Biol., 2019;0(0):. 2019
Ginsenoside Rb1 Attenuates High Glucose-Induced Oxidative Injury via the NAD-PARP-SIRT Axis in Rat Retinal Capillary Endothelial Cells
Authors: C Fan, Q Ma, M Xu, Y Qiao, Y Zhang, P Li, Y Bi, M Tang
Int J Mol Sci, 2019;20(19):. 2019
Barrier-to-autointegration factor 1 (Banf1) regulates poly [ADP-ribose] polymerase 1 (PARP1) activity following oxidative DNA damage
Authors: E Bolderson, JT Burgess, J Li, NS Gandhi, D Boucher, LV Croft, S Beard, JJ Plowman, A Suraweera, MN Adams, A Naqi, SD Zhang, DA Sinclair, KJ O'Byrne, DJ Richard
Nat Commun, 2019;10(1):5501. 2019
Enhancement of synthetic lethality via combinations of ABT-888, a PARP inhibitor, and carboplatin in vitro and in vivo using BRCA1 and BRCA2 isogenic models.
Authors: Clark C, Weitzel J, O'Connor T
Mol Cancer Ther, 0;11(9):1948-58. 0
What wavelength should be used to read the chemiluminescent plate?
The peroxyglow substrates used in the chemiluminescent kit are in the visible light range, so the plate reader should not filter the light source or the detector. The detector measures light intensity without specificity for a particular wavelength. The results are in all the wavelengths and report the amount of detected emitted light in relative light units or RLU. We recommend using a luminometer that is used for luciferase assays.
What is the maximum DMSO concentration in samples that can interfere with the assay?
Maximum DMSO concentration for inhibitor vehicles has not been evaluated. It is recommended to include a DMSO only control in the assay.
Does this kit contain the full-length or truncated form of PARP?
This is the full length PARP1 containing a short ubiquitin tag on the amino terminus.
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