Universal Chemiluminescent PARP Assay - Histone Coated Wells

For the in vitro screening of candidate PARP-1 inhibitors
Catalog # Availability Size / Price Qty
4676-096-K
Product Details
Citations (4)
FAQs
Reviews

Universal Chemiluminescent PARP Assay - Histone Coated Wells Summary

Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits

• Chemiluminescent readout
• 96 strip-well format
• Sensitive – detects 10 mU PARP /well
• Assay Time ~3 hrs

Why Use the Universal Chemiluminescent PARP Assay Kit?

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.

Product Specifications

For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents

• 3-Aminobenzamide
• Human PARP 1 Enzyme, HSA
• 20X PARP Buffer
• 10X PARP Cocktail
• 10X Activated DNA
• 10X Strep-Diluent
• PeroxyGlow™ A
• PeroxyGlow™ B
• Histone-Coated White Strip Well Plate
• Strep-HRP

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Citations for Universal Chemiluminescent PARP Assay - Histone Coated Wells

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Uncoupling of PARP1 trapping and inhibition using selective PARP1 degradation
    Authors: S Wang, L Han, J Han, P Li, Q Ding, QJ Zhang, ZP Liu, C Chen, Y Yu
    Nat. Chem. Biol., 2019;0(0):.  2019
  2. Ginsenoside Rb1 Attenuates High Glucose-Induced Oxidative Injury via the NAD-PARP-SIRT Axis in Rat Retinal Capillary Endothelial Cells
    Authors: C Fan, Q Ma, M Xu, Y Qiao, Y Zhang, P Li, Y Bi, M Tang
    Int J Mol Sci, 2019;20(19):.  2019
  3. Barrier-to-autointegration factor 1 (Banf1) regulates poly [ADP-ribose] polymerase 1 (PARP1) activity following oxidative DNA damage
    Authors: E Bolderson, JT Burgess, J Li, NS Gandhi, D Boucher, LV Croft, S Beard, JJ Plowman, A Suraweera, MN Adams, A Naqi, SD Zhang, DA Sinclair, KJ O'Byrne, DJ Richard
    Nat Commun, 2019;10(1):5501.  2019
  4. Enhancement of synthetic lethality via combinations of ABT-888, a PARP inhibitor, and carboplatin in vitro and in vivo using BRCA1 and BRCA2 isogenic models.
    Authors: Clark C, Weitzel J, O'Connor T
    Mol Cancer Ther, 0;11(9):1948-58.  0

FAQs

  1. What wavelength should be used to read the chemiluminescent plate?

    • The peroxyglow substrates used in the chemiluminescent kit are in the visible light range, so the plate reader should not filter the light source or the detector. The detector measures light intensity without specificity for a particular wavelength. The results are in all the wavelengths and report the amount of detected emitted light in relative light units or RLU. We recommend using a luminometer that is used for luciferase assays.

  2. What is the maximum DMSO concentration in samples that can interfere with the assay?

    • Maximum DMSO concentration for inhibitor vehicles has not been evaluated. It is recommended to include a DMSO only control in the assay.

  3. Does this kit contain the full-length or truncated form of PARP?

    • This is the full length PARP1 containing a short ubiquitin tag on the amino terminus. 

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