Universal Chemiluminescent PARP Assay - Histone Coated Wells

For the in vitro screening of candidate PARP-1 inhibitors
Catalog # Availability Size / Price Qty

SAVE on Cell Culture products with CULTURE20 (See Promo Details)

Product Details
Citations (4)

Universal Chemiluminescent PARP Assay - Histone Coated Wells Summary

Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits

• Chemiluminescent readout
• 96 strip-well format
• Sensitive – detects 10 mU PARP /well
• Assay Time ~3 hrs

Why Use the Universal Chemiluminescent PARP Assay Kit?

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.

Product Specifications

For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents

• 3-Aminobenzamide
• Human PARP 1 Enzyme, HSA
• 20X PARP Buffer
• 10X PARP Cocktail
• 10X Activated DNA
• 10X Strep-Diluent
• PeroxyGlow™ A
• PeroxyGlow™ B
• Histone-Coated White Strip Well Plate
• Strep-HRP


Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.


For research use only. Not for diagnostic use.

Product Datasheets

You must select a language.


Citations for Universal Chemiluminescent PARP Assay - Histone Coated Wells

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Barrier-to-autointegration factor 1 (Banf1) regulates poly [ADP-ribose] polymerase 1 (PARP1) activity following oxidative DNA damage
    Authors: E Bolderson, JT Burgess, J Li, NS Gandhi, D Boucher, LV Croft, S Beard, JJ Plowman, A Suraweera, MN Adams, A Naqi, SD Zhang, DA Sinclair, KJ O'Byrne, DJ Richard
    Nat Commun, 2019-12-03;10(1):5501.  2019-12-03
  2. Uncoupling of PARP1 trapping and inhibition using selective PARP1 degradation
    Authors: S Wang, L Han, J Han, P Li, Q Ding, QJ Zhang, ZP Liu, C Chen, Y Yu
    Nat. Chem. Biol., 2019-10-28;0(0):.  2019-10-28
  3. Ginsenoside Rb1 Attenuates High Glucose-Induced Oxidative Injury via the NAD-PARP-SIRT Axis in Rat Retinal Capillary Endothelial Cells
    Authors: C Fan, Q Ma, M Xu, Y Qiao, Y Zhang, P Li, Y Bi, M Tang
    Int J Mol Sci, 2019-10-05;20(19):.  2019-10-05
  4. Enhancement of synthetic lethality via combinations of ABT-888, a PARP inhibitor, and carboplatin in vitro and in vivo using BRCA1 and BRCA2 isogenic models.
    Authors: Clark C, Weitzel J, O'Connor T
    Mol Cancer Ther, 2012-07-09;11(9):1948-58.  2012-07-09


  1. What wavelength should be used to read the chemiluminescent plate?

    • The peroxyglow substrates used in the chemiluminescent kit are in the visible light range, so the plate reader should not filter the light source or the detector. The detector measures light intensity without specificity for a particular wavelength. The results are in all the wavelengths and report the amount of detected emitted light in relative light units or RLU. We recommend using a luminometer that is used for luciferase assays.

  2. What is the maximum DMSO concentration in samples that can interfere with the assay?

    • Maximum DMSO concentration for inhibitor vehicles has not been evaluated. It is recommended to include a DMSO only control in the assay.

  3. Does this kit contain the full-length or truncated form of PARP?

    • This is the full length PARP1 containing a short ubiquitin tag on the amino terminus. 

View all FAQs

Reviews for Universal Chemiluminescent PARP Assay - Histone Coated Wells

There are currently no reviews for this product. Be the first to review Universal Chemiluminescent PARP Assay - Histone Coated Wells and earn rewards!

Have you used Universal Chemiluminescent PARP Assay - Histone Coated Wells?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review