Universal Chemiluminescent PARP Assay - Histone Coated Wells

For the in vitro screening of candidate PARP-1 inhibitors
Catalog # Availability Size / Price Qty
4676-096-K
Product Details
Citations (4)
FAQs
Reviews

Universal Chemiluminescent PARP Assay - Histone Coated Wells Summary

Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits

• Chemiluminescent readout
• 96 strip-well format
• Sensitive – detects 10 mU PARP /well
• Assay Time ~3 hrs

Why Use the Universal Chemiluminescent PARP Assay Kit?

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.

Product Specifications

For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents

• 3-Aminobenzamide
• Human PARP 1 Enzyme, HSA
• 20X PARP Buffer
• 10X PARP Cocktail
• 10X Activated DNA
• 10X Strep-Diluent
• PeroxyGlow™ A
• PeroxyGlow™ B
• Histone-Coated White Strip Well Plate
• Strep-HRP

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for Universal Chemiluminescent PARP Assay - Histone Coated Wells

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Uncoupling of PARP1 trapping and inhibition using selective PARP1 degradation
    Authors: S Wang, L Han, J Han, P Li, Q Ding, QJ Zhang, ZP Liu, C Chen, Y Yu
    Nat. Chem. Biol., 2019;0(0):.  2019
  2. Ginsenoside Rb1 Attenuates High Glucose-Induced Oxidative Injury via the NAD-PARP-SIRT Axis in Rat Retinal Capillary Endothelial Cells
    Authors: C Fan, Q Ma, M Xu, Y Qiao, Y Zhang, P Li, Y Bi, M Tang
    Int J Mol Sci, 2019;20(19):.  2019
  3. Barrier-to-autointegration factor 1 (Banf1) regulates poly [ADP-ribose] polymerase 1 (PARP1) activity following oxidative DNA damage
    Authors: E Bolderson, JT Burgess, J Li, NS Gandhi, D Boucher, LV Croft, S Beard, JJ Plowman, A Suraweera, MN Adams, A Naqi, SD Zhang, DA Sinclair, KJ O'Byrne, DJ Richard
    Nat Commun, 2019;10(1):5501.  2019
  4. Enhancement of synthetic lethality via combinations of ABT-888, a PARP inhibitor, and carboplatin in vitro and in vivo using BRCA1 and BRCA2 isogenic models.
    Authors: Clark C, Weitzel J, O'Connor T
    Mol Cancer Ther, 0;11(9):1948-58.  0

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