PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
Universal Chemiluminescent PARP Assay - Histone Coated Wells
R&D Systems | Catalog # 4676-096-K
For the in vitro screening of candidate PARP-1 inhibitors
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Key Product Details
Features
Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.
Key Benefits
- Chemiluminescent readout
- 96 strip-well format
- Sensitive – detects 10 mU PARP /well
- Assay Time ~3 hrs
Species
Multi-Species
Product Summary for Universal Chemiluminescent PARP Assay - Histone Coated Wells
Why Use the Universal Chemiluminescent PARP Assay Kit?
This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.
Product Specifications
For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.
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Kit Contents for Universal Chemiluminescent PARP Assay - Histone Coated Wells
- 3-Aminobenzamide
- Human PARP 1 Enzyme, HSA
- 20X PARP Buffer
- 10X PARP Cocktail
- 10X Activated DNA
- 10X Strep-Diluent
- PeroxyGlow™ A
- PeroxyGlow™ B
- Histone-Coated White Strip Well Plate
- Strep-HRP
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: PARP
Long Name
Poly [ADP-ribose] Polymerase
Alternate Names
ADPRT, PARP1, PPOL
Additional PARP Products
Product Documents for Universal Chemiluminescent PARP Assay - Histone Coated Wells
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Related Research Areas
Citations for Universal Chemiluminescent PARP Assay - Histone Coated Wells
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FAQs for Universal Chemiluminescent PARP Assay - Histone Coated Wells
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Q: Does this kit contain the full-length or truncated form of PARP?
A: This is the full length PARP1 containing a short ubiquitin tag on the amino terminus.
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Q: What is the maximum DMSO concentration in samples that can interfere with the assay?
A: Maximum DMSO concentration for inhibitor vehicles has not been evaluated. It is recommended to include a DMSO only control in the assay.
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Q: What wavelength should be used to read the chemiluminescent plate?
A: The peroxyglow substrates used in the chemiluminescent kit are in the visible light range, so the plate reader should not filter the light source or the detector. The detector measures light intensity without specificity for a particular wavelength. The results are in all the wavelengths and report the amount of detected emitted light in relative light units or RLU. We recommend using a luminometer that is used for luciferase assays.
-
Q: Does this kit contain the full-length or truncated form of PARP?
A: This is the full length PARP1 containing a short ubiquitin tag on the amino terminus.
-
Q: What is the maximum DMSO concentration in samples that can interfere with the assay?
A: Maximum DMSO concentration for inhibitor vehicles has not been evaluated. It is recommended to include a DMSO only control in the assay.
-
Q: What wavelength should be used to read the chemiluminescent plate?
A: The peroxyglow substrates used in the chemiluminescent kit are in the visible light range, so the plate reader should not filter the light source or the detector. The detector measures light intensity without specificity for a particular wavelength. The results are in all the wavelengths and report the amount of detected emitted light in relative light units or RLU. We recommend using a luminometer that is used for luciferase assays.
-
Q: Does this kit contain the full-length or truncated form of PARP?
A: This is the full length PARP1 containing a short ubiquitin tag on the amino terminus.
-
Q: What is the maximum DMSO concentration in samples that can interfere with the assay?
A: Maximum DMSO concentration for inhibitor vehicles has not been evaluated. It is recommended to include a DMSO only control in the assay.
-
Q: What wavelength should be used to read the chemiluminescent plate?
A: The peroxyglow substrates used in the chemiluminescent kit are in the visible light range, so the plate reader should not filter the light source or the detector. The detector measures light intensity without specificity for a particular wavelength. The results are in all the wavelengths and report the amount of detected emitted light in relative light units or RLU. We recommend using a luminometer that is used for luciferase assays.
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