Universal Chemiluminescent PARP Assay - Histone Coated Wells
Universal Chemiluminescent PARP Assay - Histone Coated Wells Summary
• Chemiluminescent readout
• 96 strip-well format
• Sensitive – detects 10 mU PARP /well
• Assay Time ~3 hrs
Why Use the Universal Chemiluminescent PARP Assay Kit?
This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.
For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.
• Human PARP 1 Enzyme, HSA
• 20X PARP Buffer
• 10X PARP Cocktail
• 10X Activated DNA
• 10X Strep-Diluent
• PeroxyGlow™ A
• PeroxyGlow™ B
• Histone-Coated White Strip Well Plate
For research use only. Not for diagnositic use.
Citations for Universal Chemiluminescent PARP Assay - Histone Coated Wells
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Citations: Showing 1 - 4
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Uncoupling of PARP1 trapping and inhibition using selective PARP1 degradation
Authors: S Wang, L Han, J Han, P Li, Q Ding, QJ Zhang, ZP Liu, C Chen, Y Yu
Nat. Chem. Biol., 2019;0(0):. 2019
Ginsenoside Rb1 Attenuates High Glucose-Induced Oxidative Injury via the NAD-PARP-SIRT Axis in Rat Retinal Capillary Endothelial Cells
Authors: C Fan, Q Ma, M Xu, Y Qiao, Y Zhang, P Li, Y Bi, M Tang
Int J Mol Sci, 2019;20(19):. 2019
Barrier-to-autointegration factor 1 (Banf1) regulates poly [ADP-ribose] polymerase 1 (PARP1) activity following oxidative DNA damage
Authors: E Bolderson, JT Burgess, J Li, NS Gandhi, D Boucher, LV Croft, S Beard, JJ Plowman, A Suraweera, MN Adams, A Naqi, SD Zhang, DA Sinclair, KJ O'Byrne, DJ Richard
Nat Commun, 2019;10(1):5501. 2019
Enhancement of synthetic lethality via combinations of ABT-888, a PARP inhibitor, and carboplatin in vitro and in vivo using BRCA1 and BRCA2 isogenic models.
Authors: Clark C, Weitzel J, O'Connor T
Mol Cancer Ther, 0;11(9):1948-58. 0
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