![Immunohistochemistry: USF2 Antibody [NBP1-92650]](https://resources.rndsystems.com/images/products/USF2-Antibody-Immunohistochemistry-NBP1-92650-img0003.jpg "Immunohistochemistry: USF2 Antibody [NBP1-92650]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 296 and 346 of human Upstream Transcription Factor 2 using the numbering given in entry NP_003358.1 (GeneID 7392).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for USF2 Antibody - BSA Free
Immunohistochemistry: USF2 Antibody [NBP1-92650]
Immunohistochemistry: USF2 Antibody [NBP1-92650] - Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti- USF2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunoprecipitation: USF2 Antibody [NBP1-92650]](https://resources.rndsystems.com/images/products/USF2-Antibody-Immunoprecipitation-NBP1-92650-img0002.jpg "Immunoprecipitation: USF2 Antibody [NBP1-92650]")
Immunoprecipitation: USF2 Antibody [NBP1-92650]
Immunoprecipitation: USF2 Antibody [NBP1-92650] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USF2 antibody NBP1-92647 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies NBP1-92649 and NBP1-92650, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).![Immunoprecipitation: USF2 Antibody [NBP1-92650]](https://resources.rndsystems.com/images/products/USF2-Antibody-Immunoprecipitation-NBP1-92650-img0004.jpg "Immunoprecipitation: USF2 Antibody [NBP1-92650]")
Immunoprecipitation: USF2 Antibody [NBP1-92650]
Immunoprecipitation: USF2 Antibody [NBP1-92650] - Detection of human USF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USF2 antibody NBP1-92650 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies NBP1-92649 and NBP1-92647, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).Applications for USF2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: USF2
Alternate Names
BHLHB12, bHLHb12upstream stimulatory factor 2, Class B basic helix-loop-helix protein 12, FIPc-fos interacting protein, FOS-interacting protein, Major late transcription factor 2, Upstream transcription factor 2, upstream transcription factor 2, c-fos interacting
Gene Symbol
USF2
Additional USF2 Products
Product Documents for USF2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for USF2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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