![Western Blot: USF2 Antibody [NBP3-12887]](https://resources.rndsystems.com/images/products/USF2-Antibody-Western-Blot-NBP3-12887-img0003.jpg "Western Blot: USF2 Antibody [NBP3-12887]")
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (97%), Rat (97%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human USF2. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (81%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for USF2 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: USF2 Antibody [NBP3-12887]](https://resources.rndsystems.com/images/products/USF2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12887-img0002.jpg "Immunocytochemistry/ Immunofluorescence: USF2 Antibody [NBP3-12887]")
Immunocytochemistry/ Immunofluorescence: USF2 Antibody [NBP3-12887]
Immunocytochemistry/Immunofluorescence: USF2 Antibody [NBP3-12887] - USF2 antibody detects USF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USF2 stained by USF2 antibody (NBP3-12887) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.![Western Blot: USF2 Antibody [NBP3-12887]](https://resources.rndsystems.com/images/products/USF2-Antibody-Western-Blot-NBP3-12887-img0001.jpg "Western Blot: USF2 Antibody [NBP3-12887]")
Western Blot: USF2 Antibody [NBP3-12887]
Western Blot: USF2 Antibody [NBP3-12887] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with USF2 antibody (NBP3-12887) diluted at 1:500.Applications for USF2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: USF2
Alternate Names
BHLHB12, bHLHb12upstream stimulatory factor 2, Class B basic helix-loop-helix protein 12, FIPc-fos interacting protein, FOS-interacting protein, Major late transcription factor 2, Upstream transcription factor 2, upstream transcription factor 2, c-fos interacting
Gene Symbol
USF2
Additional USF2 Products
Product Documents for USF2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for USF2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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