USP14 Antibody (3A3J1)

Name: USP14 Antibody (3A3J1)
Brand: Novus Biologicals
SKU: NBP3-15796
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15796

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3A3J1 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 371-473 of human USP14 (P54578). RSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP14 Antibody (3A3J1)

Western Blot: USP14 Antibody (3A3J1) [NBP3-15796]

Western Blot: USP14 Antibody (3A3J1) [NBP3-15796] - Western blot analysis of extracts of various cell lines, using USP14 Rabbit mAb (NBP3-15796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunoprecipitation: USP14 Antibody (3A3J1) [NBP3-15796]

Immunoprecipitation: USP14 Antibody (3A3J1) [NBP3-15796] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug USP14 antibody (NBP3-15796). Western blot was performed from the immunoprecipitate using USP14 antibody (NBP3-15796) at a dilition of 1:2000.

Applications for USP14 Antibody (3A3J1)

Application

Recommended Usage

Immunoprecipitation

1:1000 - 1:5000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP14

USP14 encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Ubiquitin Specific Protease 14

Alternate Names

TGT, Ubiquitin Thioesterase 14, Ubp6

Gene Symbol

USP14

Additional USP14 Products

Product Documents for USP14 Antibody (3A3J1)

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Certificate of Analysis

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Product Specific Notices for USP14 Antibody (3A3J1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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