USP16 Antibody (OTI1B4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74805

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1B4

Format

Azide and BSA Free
View all USP16 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 137-466 of human USP16(NP_001001992) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for USP16 Antibody (OTI1B4) - Azide and BSA Free

Western Blot: USP16 Antibody (OTI1B4)Azide and BSA Free [NBP2-74805]

Western Blot: USP16 Antibody (OTI1B4)Azide and BSA Free [NBP2-74805]

Western Blot: USP16 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74805] - E.coli lysate (5 ug, left lane) and E.coli lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 137-466 of human USP16(NP_001001992) were separated by SDS-PAGE and immunoblotted with USP16.
Western Blot: USP16 Antibody (OTI1B4)Azide and BSA Free [NBP2-74805]

Western Blot: USP16 Antibody (OTI1B4)Azide and BSA Free [NBP2-74805]

Western Blot: USP16 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74805] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Applications for USP16 Antibody (OTI1B4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:200-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP16

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 16 (USP16) is a member of this family. USP16 activity is cell cycle regulated via phosphorylation and participates in cell cycle control by influencing mitotic chromosome condensation and histone 2A (H2A) deubiquitination.

Long Name

Ubiquitin Specific Protease 16

Alternate Names

UBP-M

Gene Symbol

USP16

Additional USP16 Products

Product Documents for USP16 Antibody (OTI1B4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for USP16 Antibody (OTI1B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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