UT2/SLC14A2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-44375

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UT2/SLC14A2 Primary Antibodies »

Product Specifications

Immunogen

Produced against a synthetic peptide mapped to the C-terminal tail (amino acids 911-929) of rat UT-A1 (antibody designation L194)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 30101448).

Localization

Cell Membrane, Apical Cell Membrane

Specificity

Detects approx 97 and 127kDa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UT2/SLC14A2 Antibody - BSA Free

Western Blot: UT2/SLC14A2 Antibody [NBP1-44375]

Western Blot: UT2/SLC14A2 Antibody [NBP1-44375]

Western Blot: UT2/SLC14A2 Antibody [NBP1-44375] - Western blot analysis of Rat Inner medulla showing detection of UT2/SLC14A2 protein using Rabbit Anti-UT2/SLC14A2 Polyclonal Antibody (NBP1-44375). Primary Antibody: Rabbit Anti-UT2/SLC14A2 Polyclonal Antibody (NBP1-44375) at 1:1000.
Immunohistochemistry: UT2/SLC14A2 Antibody [NBP1-44375]

Immunohistochemistry: UT2/SLC14A2 Antibody [NBP1-44375]

Immunohistochemistry: UT2/SLC14A2 Antibody [NBP1-44375] - Immunohistochemistry analysis using Rabbit Anti-UT2/SLC14A2 Polyclonal Antibody (NBP1-44375). Tissue: kidney tissue. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-UT2/SLC14A2 Polyclonal Antibody (NBP1-44375) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C)UT2/SLC14A2 Antibody (D) Composite.
Immunohistochemistry-Paraffin: UT2/SLC14A2 Antibody [NBP1-44375]

Immunohistochemistry-Paraffin: UT2/SLC14A2 Antibody [NBP1-44375]

Immunohistochemistry-Paraffin: UT2/SLC14A2 Antibody [NBP1-44375] - kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-UT-A1 Polyclonal Antibody (NBP1-44375) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C)UT-A1 Antibody (D) Composite.

Applications for UT2/SLC14A2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunohistochemistry

1:25

Western Blot

1:1000
Application Notes
1 ug/ml of UT-A1 Antibody was sufficient for detection of UT-A1 in 20 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UT2/SLC14A2

UT-A1, a kidney-specific urea transporter is expressed in the renal collecting duct where it mediates transepithelial urea transport and is a target for regulation by vasopressin. Urea movement out of the collecting duct in the inner medulla of the kidney allows accumulation of urea in the medullary interstitium, thereby allowing maximum water reabsorption from the collecting ducts. (The antibody also recognizes a second protein from the UT-A gene driven by transcription from an alternative promotor and expressed in the thin descending limb of Henle, viz. UT-A2.) (1, 2)

Long Name

Urea Transporter 2

Alternate Names

SLC14A2, UT-A2, UTA

Entrez Gene IDs

27411 (Mouse); 54302 (Rat)

Gene Symbol

SLC14A2

UniProt

Q62668 (Rat)

Additional UT2/SLC14A2 Products

Product Documents for UT2/SLC14A2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for UT2/SLC14A2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UT2/SLC14A2 Antibody - BSA Free

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