V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-37825

Novus Biologicals

Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, ELISA, Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # E10/V4RR

Format

Azide and BSA Free
View all V5 Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH

Specificity

This product recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins. It has successfully been used in immunoprecipitation, ELISA, Western blotting, and immunofluorescence applications.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free

Western Blot: V5 Epitope Tag Antibody (E10/V4RR)Azide and BSA Free [NBP2-37825]

Western Blot: V5 Epitope Tag Antibody (E10/V4RR)Azide and BSA Free [NBP2-37825]

Western Blot: V5 Epitope Tag Antibody (E10/V4RR) [NBP2-37825] - Analysis of 15ug of 293T cell lysates, from cells transfected with a V5-Wnt3a or V5-Vstm2 construct for the indicated number of hours, per well. Data courtesy of the Innovators Program.
Western Blot: V5 Epitope Tag Antibody (E10/V4RR)Azide and BSA Free [NBP2-37825]

Western Blot: V5 Epitope Tag Antibody (E10/V4RR)Azide and BSA Free [NBP2-37825]

Western Blot: V5 Epitope Tag Antibody (E10/V4RR) [NBP2-37825] - Analysis of 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2).

Applications for V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunofluorescence

1:500 - 1:2000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:1000 - 1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: V5 Epitope Tag

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Alternate Names

GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5

Additional V5 Epitope Tag Products

Product Documents for V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for V5 Epitope Tag Antibody (E10/V4RR) - Azide and BSA Free

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Protocols

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FAQs

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