VAChT/SLC18A3 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB100-91347

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all VAChT/SLC18A3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from human VAChT/SLC18A3 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for VAChT/SLC18A3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

10-50 ug/ml

Western Blot

10-50 ug/ml
Application Notes
Use in ICC/IF reported in scientific publication (PMID: 23658180).

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Lyophilized from PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: VAChT/SLC18A3

Vesicular Acetylcholine Transporter (VAChT), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimers disease and other nervous system disorders.

Long Name

Vesicular Acetylcholine Transporter/Solute Carrier Family 18 Member 3

Alternate Names

SLC18A3

Entrez Gene IDs

6572 (Human); 20508 (Mouse); 60422 (Rat)

Gene Symbol

SLC18A3

UniProt

Q16572 (Human)

Additional VAChT/SLC18A3 Products

Product Documents for VAChT/SLC18A3 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VAChT/SLC18A3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for VAChT/SLC18A3 Antibody - Azide and BSA Free

Customer Reviews for VAChT/SLC18A3 Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review VAChT/SLC18A3 Antibody - Azide and BSA Free and earn rewards!

Have you used VAChT/SLC18A3 Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VAChT/SLC18A3 Antibody - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I have a question about the immunogen regions of NB100-91347, NB100-91348 and NB110-74764.

    A: NB100-91348 and NB100-91347 have the same immunogen region, 430-530aa. NB100-91347 is an IgG purified form of NB1100-91348. NB110-74764 comes from the same region, but has a different immunogen than the other two.

  • Q: Would it be possible for you to please let me know the epitopes for NB100-91347?

    A: The epitope for NB100-91347 is between aa region 450-505 of human VACHT raised in rabbit (IgG purified).

  • Q: I have a question about the immunogen regions of NB100-91347, NB100-91348 and NB110-74764.

    A: NB100-91348 and NB100-91347 have the same immunogen region, 430-530aa. NB100-91347 is an IgG purified form of NB1100-91348. NB110-74764 comes from the same region, but has a different immunogen than the other two.

  • Q: Would it be possible for you to please let me know the epitopes for NB100-91347?

    A: The epitope for NB100-91347 is between aa region 450-505 of human VACHT raised in rabbit (IgG purified).

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies