VasoTACS In Situ Apoptosis Detection Kit
VasoTACS In Situ Apoptosis Detection Kit Summary
• Performance tested on vascular tissues.
• Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
• Helps resolve unique problems encountered when detecting apoptotic vascular cells.
Why Use the VasoTACS In Situ Apoptosis Detection Kit?
This kit allows the user to successfully label apoptotic cells, including endothelial and smooth muscle cells, throughout the vascular system. Similar to the CardioTACS Kit, this kit is also based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label detection system with Red Counterstain C. Specifically, VasoTACS™ has been tested and optimized to help resolve the unique problems encountered when detecting apoptotic vascular cells.
• Proteinase K
• TACS-Blue Label
• Blue Strep-HRP Diluent
• TACS-Nuclease Buffer
• Red Counterstain C
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Manganese Cation
For research use only. Not for diagnositic use.
Visualization of Apoptotic Cells in Vascular Tissue using the VasoTACS In Situ Apoptosis Detection Kit. TIssue from the small artery of rat was formalin-fixed and paraffin-embedded following the initiation of drug-induced apoptosis. The tissue was processed according to the protocol in the VasoTACS Kit (Catalog # 4826-30-K) and visualized by light microscopy. Brown stained nuclei following counterstaining with Red Counterstain C indicated apoptotic cells. Data was provided courtesy of Dr. Jun Zhang, FDA.
Citations for VasoTACS In Situ Apoptosis Detection Kit
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Dexamethasone induces apoptosis in pulmonary arterial smooth muscle cells.
Authors: Price L, Shao D, Meng C, Perros F, Garfield B, Zhu J, Montani D, Dorfmuller P, Humbert M, Adcock I, Wort S
Respir Res, 2015;16(0):114. 2015
The Down syndrome critical region gene 1 short variant promoters direct vascular bed-specific gene expression during inflammation in mice.
Authors: Minami T, Yano K, Miura M, Kobayashi M, Suehiro J, Reid PC, Hamakubo T, Ryeom S, Aird WC, Kodama T
J. Clin. Invest., 2009;119(8):2257-70. 2009
Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells.
Authors: Song H, Suehiro J, Kanki Y, Kawai Y, Inoue K, Daida H, Yano K, Ohhashi T, Oettgen P, Aird WC, Kodama T, Minami T
J. Biol. Chem., 2009;284(42):29109-24. 2009
Endothelial lipid phosphate phosphatase-3 deficiency that disrupts the endothelial barrier function is a modifier of cardiovascular development.
Authors: Chatterjee I, Baruah J, Lurie E, Wary K
Cardiovasc Res, 0;111(1):105-18. 0
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