VasoTACS In Situ Apoptosis Detection Kit

R&D Systems | Catalog # 4826-30-K

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded vascular cells and tissues.
R&D Systems
100% Guarantee

Key Product Details

Features

Provides an effective in situ method for identifying apoptosis in vascular samples using an optimized TUNEL based assay.

Key Benefits

  • Performance tested on vascular tissues.
  • Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents.
  • Helps resolve unique problems encountered when detecting apoptotic vascular cells.

Species

Multi-Species

Product Summary for VasoTACS In Situ Apoptosis Detection Kit

Why Use the VasoTACS In Situ Apoptosis Detection Kit?
This kit allows the user to successfully label apoptotic cells, including endothelial and smooth muscle cells, throughout the vascular system. Similar to the CardioTACS Kit, this kit is also based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label detection system with Red Counterstain C. Specifically, VasoTACS™ has been tested and optimized to help resolve the unique problems encountered when detecting apoptotic vascular cells.

Scientific Data Images for VasoTACS In Situ Apoptosis Detection Kit

VasoTACS In Situ Apoptosis Detection Kit_Rat Artery Tissue

Visualization of Apoptotic Cells in Vascular Tissue using the VasoTACS In Situ Apoptosis Detection Kit.

TIssue from the small artery of rat was formalin-fixed and paraffin-embedded following the initiation of drug-induced apoptosis. The tissue was processed according to the protocol in the VasoTACS Kit (Catalog # 4826-30-K) and visualized by light microscopy. Brown stained nuclei following counterstaining with Red Counterstain C indicated apoptotic cells. Data was provided courtesy of Dr. Jun Zhang, FDA.

Kit Contents for VasoTACS In Situ Apoptosis Detection Kit

  • Proteinase K
  • Streptavidin-HRP
  • TACS-Blue Label
  • Blue Strep-HRP Diluent
  • TACS-Nuclease
  • TACS-Nuclease Buffer
  • Red Counterstain C
  • TACS 2 TdT Labeling Buffer
  • TACS 2 TdT Stop Buffer
  • TACS 2 TdT dNTP
  • TdT Enzyme
  • 50x Manganese Cation
  • Cytonin

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Documents for VasoTACS In Situ Apoptosis Detection Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Citations for VasoTACS In Situ Apoptosis Detection Kit

Customer Reviews for VasoTACS In Situ Apoptosis Detection Kit

There are currently no reviews for this product. Be the first to review VasoTACS In Situ Apoptosis Detection Kit and earn rewards!

Have you used VasoTACS In Situ Apoptosis Detection Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Activity Assays