Human Vav-1 ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Kit Contents for Human Vav-1 ELISA Kit (Colorimetric)

• Assay Diluent A
• Assay Diluent B
• Detection Reagent A
• Detection Reagent B
• Instruction manual
• Plate sealer for 96 wells
• Pre-coated ready to use 96-well strip plate
• Standard
• Standard Diluent
• Stop Solution
• TMB Substrate
• Wash Buffer (30 x concentrate)

Preparation and Storage

Background: Vav-1

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels (2). As a signaling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein (3). Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response (2).

Additional Vav-1 Products

• All Products for Vav-1
• Vav-1 ELISA Kits
• Vav-1 Lysates
• Vav-1 Primary Antibodies
• Vav-1 Proteins and Enzymes

Product Documents for Human Vav-1 ELISA Kit (Colorimetric)