VE-Cadherin [p Tyr731] Antibody - BSA Free

Name: VE-Cadherin [p Tyr731] Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-54766
Price: 599 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-54766

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all VE-Cadherin Primary Antibodies »

Product Specifications

Immunogen

VE-Cadherin (phospho Tyr731) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine731 (H-I-Y (p)-G-Y) derived from Human VE-Cadherin.

Modification

p Tyr731

Specificity

The antibody detects endogenous level of VE-Cadherin only when phosphorylated at tyrosine 731.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VE-Cadherin [p Tyr731] Antibody - BSA Free

Western Blot: VE-Cadherin [p Tyr731] AntibodyBSA Free [NBP2-54766]

Western Blot: VE-Cadherin [p Tyr731] Antibody [NBP2-54766] - Analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) Antibody. The lane on the right is treated with the antigen-specific peptide.

Applications for VE-Cadherin [p Tyr731] Antibody - BSA Free

Application

Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Epitope affinity purified

Formulation

PBS (pH 7.4) (without Mg2+ and Ca2+), 150mM NaCl, 50% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 °C.

Background: VE-Cadherin

VE-cadherin (cadherin-5) is an endothelial specific adhesion molecule that is essential for the maintenance of endothelial barrier function and angiogenesis. VE-cadherin is linked to the actin cytoskeleton through a number of adaptor proteins including a-, â-, and ã-catenin. Cytoskeletal dynamics and phosphorylation regulate VE-cadherin mediated cell-cell adhesion. The cytosolic C-terminal Src kinase (Csk) binds via its SH2 domain to the phosphorylated tyrosine 658 site of VE-cadherin. This interaction regulates density dependent cell proliferation. Phosphorylation of tyrosine 658 has also been shown to lead to the uncoupling of p120-catenin from the cytoplasmic tail of VE-cadherin. This phosphorylation event is sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Gene Symbol

CDH5

Additional VE-Cadherin Products

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Product Specific Notices for VE-Cadherin [p Tyr731] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.