Human Vitronectin ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP3-07112

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.56 ng/mL

Assay Range

0.94 - 60 ng/mL
View all Vitronectin ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

This kit recognizes Human VTN in samples. No significant cross-reactivity or interference between Human Vitronectin and analogues was observed.

Recovery for Human Vitronectin ELISA Kit (Chemiluminescence)

Recovery

92 - 116%

Linearity

Human Vitronectin ELISA Kit (Chemiluminescence)

Scientific Data Images for Human Vitronectin ELISA Kit (Chemiluminescence)

Human Vitronectin ELISA Kit (Chemiluminescence)

ELISA: Human Vitronectin ELISA Kit (Chemiluminescence) [NBP3-07112] -

ELISA: Human Vitronectin ELISA Kit (Chemiluminescence) [NBP3-07112] - Standard Curve Reference

Kit Contents for Human Vitronectin ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Vitronectin

Monoclonal antibody BV1 recognizes human vitronectin. Vitronectin is an abundant glycoprotein (~75 kDa), consisting of 459 amino acids. About one third of the protein molecular mass is composed of carbohydrates. Vitronectin is found in blood plasma and the extracellular matrix. Vitronectin is a multifunctional protein, since it promotes attachment and spreading of animal cells in vitro, it inhibits cytolysis by the complement C5b-9 complex, and it modulates antithrombin III-thrombin action in blood coagulation. The protein consists of three domains: the N-terminal Somatomedin B domain (1-39), a central domain with hemopexin homology (131-342) and a C-terminal domain (347-459) also with hemopexin homology. The Somatomedin B domain binds to Plasminogen Activator Inhibitor-1 (PAI-1) and is responsible for PAI-1 stabilization. Furthermore, the Somatomedin B domain can also interact with the urokinase plasminogen activator receptor (uPAR). Vitronectin-uPAR interaction is required and sufficient to initiate downstream changes in cell morphology, migration and signal transduction. High plasma levels of both PAI-1 and uPAR have been shown to correlate with a negative prognosis for cancer patients. Additionally, vitronectin is a component of platelets and is as such involved in hemostasis. Amino acid 45-47 (RGD) are capable of binding to membrane bound integrins, which serve to anchor cells to the extracellular matrix. Vitronectin in plasma is an inactive monomer form. In contrast, tissue vitronectin is an active multimeric form and is able to interact with various matrix ligands like proteoglycans and collagen. Mice with a genetic deletion of vitronectin show delayed wound healing, suggesting an important role of vitronectin in tissue remodeling after injury.

Alternate Names

Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN

Gene Symbol

VTN

Additional Vitronectin Products

Product Documents for Human Vitronectin ELISA Kit (Chemiluminescence)

Certificate of Analysis

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Product Specific Notices for Human Vitronectin ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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