Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VPS16 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human VPS16 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for VPS16 Antibody - BSA Free

Western Blot: VPS16 Antibody [NBP2-97757]

Western Blot: VPS16 Antibody [NBP2-97757]

Western Blot: VPS16 Antibody [NBP2-97757] - Anti-VPS16 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 95 kDa. Observed band size: 95 kDa
Immunoprecipitation: VPS16 Antibody [NBP2-97757]

Immunoprecipitation: VPS16 Antibody [NBP2-97757]

Immunoprecipitation: VPS16 Antibody [NBP2-97757] - VPS16 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-VPS16 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-VPS16 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 95 kDa. Observed band size: 95 kDa

Applications for VPS16 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS16

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinctorganelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved invesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian classC Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, maymediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding differentisoforms have been found for this gene. (provided by RefSeq)

Alternate Names

hVPS16, vacuolar protein sorting 16 homolog (S. cerevisiae), vacuolar protein sorting protein 16, vacuolar protein sorting-associated protein 16 homolog

Gene Symbol

VPS16

Additional VPS16 Products

Product Documents for VPS16 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for VPS16 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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