VPS18 Antibody (1W8T9)
Novus Biologicals | Catalog # NBP3-15542
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1W8T9 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VPS18 (Q9P253). MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSLVVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for VPS18 Antibody (1W8T9)
Western Blot: VPS18 Antibody (1W8T9) [NBP3-15542]
Western Blot: VPS18 Antibody (1W8T9) [NBP3-15542] - Western blot analysis of extracts of various cell lines, using VPS18 Rabbit mAb (NBP3-15542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western Blot: VPS18 Antibody (1W8T9) [NBP3-15542]
Western Blot: VPS18 Antibody (1W8T9) [NBP3-15542] - Western blot analysis of extracts of Rat brain, using VPS18 Rabbit mAb (NBP3-15542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Applications for VPS18 Antibody (1W8T9)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: VPS18
Alternate Names
hVPS18, KIAA1475PEP3, vacuolar protein sorting 18 homolog (S. cerevisiae), vacuolar protein sorting protein 18, vacuolar protein sorting-associated protein 18 homolog
Gene Symbol
VPS18
Additional VPS18 Products
Product Documents for VPS18 Antibody (1W8T9)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VPS18 Antibody (1W8T9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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