VPS18 Antibody (1W8T9)

Novus Biologicals | Catalog # NBP3-15542

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1W8T9 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VPS18 (Q9P253). MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSLVVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS18 Antibody (1W8T9)

Western Blot: VPS18 Antibody (1W8T9) [NBP3-15542]

Western Blot: VPS18 Antibody (1W8T9) [NBP3-15542]

Western Blot: VPS18 Antibody (1W8T9) [NBP3-15542] - Western blot analysis of extracts of various cell lines, using VPS18 Rabbit mAb (NBP3-15542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Western Blot: VPS18 Antibody (1W8T9) [NBP3-15542]

Western Blot: VPS18 Antibody (1W8T9) [NBP3-15542]

Western Blot: VPS18 Antibody (1W8T9) [NBP3-15542] - Western blot analysis of extracts of Rat brain, using VPS18 Rabbit mAb (NBP3-15542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Applications for VPS18 Antibody (1W8T9)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS18

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Alternate Names

hVPS18, KIAA1475PEP3, vacuolar protein sorting 18 homolog (S. cerevisiae), vacuolar protein sorting protein 18, vacuolar protein sorting-associated protein 18 homolog

Gene Symbol

VPS18

Additional VPS18 Products

Product Documents for VPS18 Antibody (1W8T9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for VPS18 Antibody (1W8T9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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