VPS45 Antibody

Novus Biologicals | Catalog # NB100-41372

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-ESSQVTSRSASRR corresponding to C-Terminus according to NP_009190.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VPS45 Antibody

Western Blot: VPS45 Antibody [NB100-41372]

Western Blot: VPS45 Antibody [NB100-41372]

Western Blot: VPS45 Antibody [NB100-41372] - Staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372]

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Placenta.Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for VPS45 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 60 kDa band observed in human testis and peripheral blood mononucleocyte lysates (calculated MW of 65.1 kDa band according to NP_009190.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS45

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators.

Alternate Names

H1, H1VPS45, hlVps45, h-vps45, leucocyte vacuolar protein sorting 45, vacuolar protein sorting 45 homolog (S. cerevisiae), vacuolar protein sorting 45A, vacuolar protein sorting 45A (yeast homolog), vacuolar protein sorting 45A (yeast), vacuolar protein sorting-associated protein 45, VPS45A, VPS45AVPS54A, VPS45B, VSP45, VSP45A

Entrez Gene IDs

11311 (Human); 22365 (Mouse)

Gene Symbol

VPS45

Additional VPS45 Products

Product Documents for VPS45 Antibody

Certificate of Analysis

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Product Specific Notices for VPS45 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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