VSTM1 Antibody (1A5) - Azide Free

Novus Biologicals | Catalog # NBP3-11296

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A5

Format

Azide Free
View all VSTM1 Primary Antibodies »

Product Specifications

Immunogen

Purified VSTM1-hIg fusion protein in PBS

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<24 EU/mg

Applications for VSTM1 Antibody (1A5) - Azide Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunoprecipitation, Flow Cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: VSTM1

The monoclonal antibody 1A5 recognizes human Signal inhibitory Receptor on Leucocytes-1 (SIRL-1). In order to prevent host tissue damage immune activation has to be tighly regulated. Neutrophils and other myeloid cells have a pivotal role in controlling infection. They form the primary response at site of infection as they regulate replication of pathogens by phagocytosis, secretion of antimicrobial peptides and proinflammatory cytokines to activate the immune system. Without regulation these mediators can cause tissue damage or worse. To balance the immune response immune cells can express inhibitory receptors. The family of immune inhibitory receptors is still expending. The relatively new receptor SIRL-1 is an immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing membrane protein expressed on human phagocytes. SIRL-1 contains two ITIMs in the intracellular tail, which are crucial for function. SIRL-1 can recruit the SH2-domain containing tyrosine phosphatases, SHP-1 and SHP-2, which may mediate the inhibitory effect. ITIM-bearing receptors inhibit signals relayed by immunoreceptor tyrosine-based activating motifs (ITAMs). ITAMs are the principal mediators of signal transduction for various receptors, including FcRs and C-type lectins. The ligation of SIRL-1 dampens Fc receptor induced ROS production in phagocytes, thereby impairing the microbicidal activity of neutrophils without affecting phagocytosis. Infectious stimuli cause rapid downregulation of SIRL-1 expression. This allows microbial killing and clearance of pathogens. Neutrophil extracellular traps (NETs) have been implicated in the pathogenesis of systemic lupus erythematosus (SLE). Using anti SIRL-1 antibodies both spontaneous anti-neutrophil antibody induced NET formation is suppressed. This suggests a regulatory role for SIRL-1 in NET formation and a therapeutic target for regulation of SLE.

Long Name

V-Set And Transmembrane Domain Containing 1

Alternate Names

LAIR Homolog, OSCAR-Like Transcript-1, SIRL1, VSTM1-v1, VSTM1-v2

Gene Symbol

VSTM1

Additional VSTM1 Products

Product Documents for VSTM1 Antibody (1A5) - Azide Free

Certificate of Analysis

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Product Specific Notices for VSTM1 Antibody (1A5) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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