WDR79 Antibody (1F12) - Azide and BSA Free

Novus Biologicals | Catalog # H00055135-M04

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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F12

Format

Azide and BSA Free
View all WDR79 Primary Antibodies »

Product Specifications

Immunogen

WDR79 (NP_060551.1, 62 a.a. ~ 160 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AVSQELREGDPVSLSTPLETEFGSPSELSPRIEEQELSENTSLPAEEANGSLSEEEANGPELGSGKAMEDTSGEPAAEDEGDTAWNYSFSQLPRFLSGS

Specificity

WDR79 - WD repeat domain 79 (1F12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for WDR79 Antibody (1F12) - Azide and BSA Free

Western Blot: WDR79 Antibody (1F12) [H00055135-M04]

Western Blot: WDR79 Antibody (1F12) [H00055135-M04]

Western Blot: WDR79 Antibody (1F12) [H00055135-M04] - Analysis of WDR79 expression in A-431 (Cat # L015V1).
Immunocytochemistry/ Immunofluorescence: WDR79 Antibody (1F12) [H00055135-M04]

Immunocytochemistry/ Immunofluorescence: WDR79 Antibody (1F12) [H00055135-M04]

Immunocytochemistry/Immunofluorescence: WDR79 Antibody (1F12) [H00055135-M04] - Analysis of monoclonal antibody to WDR79 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: WDR79 Antibody (1F12) [H00055135-M04]

ELISA: WDR79 Antibody (1F12) [H00055135-M04]

ELISA: WDR79 Antibody (1F12) [H00055135-M04] - Detection limit for recombinant GST tagged WDR79 is 0.03 ng/ml as a capture antibody.

Applications for WDR79 Antibody (1F12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against cell lysate and Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in immunprecipitation reported in scientific literature (PMID 25512560)

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WDR79

WD-repeat domain 79 (WDR79) contains six WD (tryptophan-aspartate) repeat domains found in a number of proteins that function as adaptor molecules in signal transduction and cytoskeletal organization. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. The function of the WDR79 protein has not been characterized, however significant and consistent single nucleotide polymorphisms in the WDR79 gene have been found to be associated with ER negative breast cancer.

Alternate Names

FLJ10385, TCAB1WDR79telomerase Cajal body protein 1, WD repeat containing, antisense to TP53, WD repeat domain 79, WD repeat-containing protein 79, WD40 protein Wrap53, WD40 repeat-containing protein encoding RNA antisense to p53, WD-encoding RNA antisense to p53

Entrez Gene IDs

55135 (Human)

Gene Symbol

WRAP53

UniProt

NP_060551 (Human)

Additional WDR79 Products

Product Documents for WDR79 Antibody (1F12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for WDR79 Antibody (1F12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for WDR79 Antibody (1F12) - Azide and BSA Free

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Protocols

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FAQs for WDR79 Antibody (1F12) - Azide and BSA Free

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  • Q: Could you tell me if this ab can be used for IHC?

    A: Product H00055135-M04 has not yet been tested in IHC. The antibody is validated for WB, ELISA, ICC/IF, IP, and S-ELISA, so there is a good chance it might also work in IHC. If you do decide to try this antibody in your experiment, you can utilize our Innovator's Reward Program. Our Innovator’s Reward™ program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply purchase the product as normal and submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product to use in a future purchase of your choice. Reviews may also be submitted by emailing innovators@novusbio.com.

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