WIBG Recombinant Protein Antigen

Novus Biologicals | Catalog # NBP1-92596PEP

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Antibody Competition
View all WIBG Proteins and Enzymes »

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human WIBG.

Source: E. coli

Amino Acid Sequence: KEKRRQQQEKGEAEALSRTLDKVSLEETAQLPSAPQGSRAAPTAASDQPDSAATTEKAKKIKNLK

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-92596.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation, and Storage

NBP1-92596PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: WIBG

Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstreamof the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes anddirects post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) ortranslation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disruptingmature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents atranslation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have beentranslated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. Maybind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, whilePubMed:19410547 did not detect RNA-binding activity independently of the EJC

Alternate Names

MGC13064, partner of Y14 and mago, PYMProtein wibg homolog, within bgcn homolog (Drosophila)

Gene Symbol

PYM1

Additional WIBG Products

Product Documents for WIBG Recombinant Protein Antigen

Certificate of Analysis

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Product Specific Notices for WIBG Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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