WIF-1 Antibody (JE41-39)

Novus Biologicals | Catalog # NBP3-33102

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE41-39 expressed in HEK293
View all WIF-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human WIF-1 aa 330-379 / 379. (Uniprot: Q9Y5W5)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WIF-1 Antibody (JE41-39)

WIF-1 Antibody (JE41-39)

Western Blot: WIF-1 Antibody (JE41-39) [NBP3-33102]

Western Blot: WIF-1 Antibody (JE41-39) [NBP3-33102] - Western blot analysis of WIF-1 on different lysates with Rabbit anti-WIF-1 antibody (NBP3-33102) at 1/1,000 dilution.

Lane 1: HT-29 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: SW480 cell lysate (20 ug/Lane)
Lane 4: Caco-2 cell lysate (20 ug/Lane)
Lane 5: NIH/3T3 cell lysate (20 ug/Lane)
Lane 6: Mouse heart tissue lysate (40 ug/Lane)
Lane 7: Mouse lung tissue lysate (40 ug/Lane)
Lane 8: Rat lung tissue lysate (40 ug/Lane)
Lane 9: Human lung tissue lysate (40 ug/Lane)

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33102) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Applications for WIF-1 Antibody (JE41-39)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WIF-1

WNT proteins are extracellular signaling molecules involved in the control of embryonic development. This gene encodes a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals. (provided by RefSeq)

Long Name

Wnt Inhibitory Factor

Alternate Names

WIF1

Gene Symbol

WIF1

Additional WIF-1 Products

Product Documents for WIF-1 Antibody (JE41-39)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for WIF-1 Antibody (JE41-39)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for WIF-1 Antibody (JE41-39)

There are currently no reviews for this product. Be the first to review WIF-1 Antibody (JE41-39) and earn rewards!

Have you used WIF-1 Antibody (JE41-39)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies