WIPI2 Antibody (2A2)

Novus Biologicals | Catalog # NBP3-07609

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A2
View all WIPI2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 418-436) of human WIPI2 protein (exact sequence is proprietary) (Uniprot: Q9Y4P8)

Localization

Preautophagosomal structure membrane.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08559)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for WIPI2 Antibody (2A2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: WIPI2

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Alternate Names

ATG18B, Atg21, CGI-50, DKFZP434J154, DKFZp686P02188, FLJ12979, FLJ14217, FLJ42984, WD repeat domain phosphoinositide-interacting protein 2, WD repeat domain, phosphoinositide interacting 2, WD40 repeat protein interacting with phosphoinositides 2, WIPI-2, WIPI49-like protein 2

Gene Symbol

WIPI2

UniProt

Q9Y4P8 (Human)

Additional WIPI2 Products

Product Documents for WIPI2 Antibody (2A2)

Certificate of Analysis

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Product Specific Notices for WIPI2 Antibody (2A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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