Key Product Details

Species Reactivity

Human

Applications

ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)

Label

DyLight 755 (Excitation = 754 nm, Emission = 776 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 213609
View all WISP-1/CCN4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human WISP-1/CCN4
Thr23-Asn367
Accession # O95388

Specificity

Detects human WISP-1 in ELISAs.  In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Biglycan, rhDecorin, rhNOV, or rhWISP-3 was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for WISP-1/CCN4 Antibody (213609) [Dylight 755]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: WISP-1/CCN4

WISP1 encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Long Name

Wnt-1 Induced Secreted Protein 1

Alternate Names

CCN4, WISP1

Gene Symbol

WISP1

Additional WISP-1/CCN4 Products

Product Documents for WISP-1/CCN4 Antibody (213609) [Dylight 755]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for WISP-1/CCN4 Antibody (213609) [Dylight 755]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for WISP-1/CCN4 Antibody (213609) [Dylight 755]

There are currently no reviews for this product. Be the first to review WISP-1/CCN4 Antibody (213609) [Dylight 755] and earn rewards!

Have you used WISP-1/CCN4 Antibody (213609) [Dylight 755]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies