WISP3 Antibody - Azide and BSA Free

Western Blot: WISP3 AntibodyAzide and BSA Free [NBP2-93872]

Western Blot: WISP3 Antibody [NBP2-93872] - Analysis of extracts of various cell lines, using WISP3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western Blot: WISP3 Antibody - Azide and BSA Free [NBP2-93872] -

OTUB1 expression is positively correlated with CCN6 in clinical samples. (A) An invasive ductal carcinoma (Grade II, moderately differentiated) sample (T) and the adjacent non‐tumour tissue (N) were analysed with H&E staining and immunohistochemistry for OTUB1 and CCN6 (scale bar = 100 μm). (B) A breast intraductal papilloma sample (T) and the adjacent non‐tumour tissue (N) were analysed with H&E staining and immunohistochemistry for OTUB1 and CCN6 (scale bar = 100 μm). (C) Clinical samples were analysed with Western blot for OTUB1, CCN6 and GAPDH. (D) Correlation between the expression of OTUB1 and CCN6 in tumour tissues (upper panel) and adjacent non‐tumour tissues (lower panel). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37608493), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: WISP3 Antibody - Azide and BSA Free [NBP2-93872] -

OTUB1 inhibits breast cancer growth in vivo. (A) Control, OTUB1−/− and OTUB1−/− + CCN6 4T1 cells were injected into nude mice and tumour volume was measured (n = 6 for all groups) (mean +/- SEM, NC vs. OTUB1−/−: *p <.05, **p <.01; NC vs. OTUB1−/− + CCN6: #p <.05, ##p <.01). On day 9 after tumour inoculation, mice were sacrificed and tumours were collected. Tumour (B) size and (C) weight were measured (n = 6 for all groups) (mean +/- SEM, *p <.05, **p <.01). (D) Whole‐cell lysates of tumour samples were analysed by Western blot for OTUB1, CCN6 and GAPDH. (E) Tumour samples were stained for Ki‐67 and immunoreactivity was detected by 3,3′‐diaminobenzidine chromogen (brown). Sections were counterstained with haematoxylin (blue) (scale bar = 100 μm). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37608493), licensed under a CC-BY license. Not internally tested by Novus Biologicals.