![Western Blot: XPA Antibody [NB100-93321]](https://resources.rndsystems.com/images/products/XPA-Antibody-Western-Blot-NB100-93321-img0009.jpg "Western Blot: XPA Antibody [NB100-93321]")
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human xeroderma pigmentosum, complementation group A using the numbering given in entry NP_000371.1 (GeneID 7507).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for XPA Antibody
Western Blot: XPA Antibody [NB100-93321]
Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (50 ug) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321]](https://resources.rndsystems.com/images/products/XPA-Antibody-Immunohistochemistry-NB100-93321-img0006.jpg "Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321]")
Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321]
Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321] - Detection of human XPA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XPA (NB100-93321). Detection: DAB.![Immunoprecipitation: XPA Antibody [NB100-93321]](https://resources.rndsystems.com/images/products/XPA-Antibody-Immunoprecipitation-NB100-93321-img0007.jpg "Immunoprecipitation: XPA Antibody [NB100-93321]")
Immunoprecipitation: XPA Antibody [NB100-93321]
Immunoprecipitation: XPA Antibody [NB100-93321] - Detection of human XPA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XPA antibody NB100-93321 (lot NB100-93321-3) used for IP at 6 ug per reaction. XPA was also immunoprecipitated by a previous lot of this antibody (lot NB100-93321-2) and another rabbit anti-XPA antibody. For blotting immunoprecipitated XPA, NB100-93321 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for XPA Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: XPA
Long Name
Xeroderma Pigmentosum, Complementation Group A
Alternate Names
XP1
Entrez Gene IDs
7507 (Human)
Gene Symbol
XPA
UniProt
Additional XPA Products
Product Documents for XPA Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for XPA Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for XPA Antibody
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Q: What's the size of protein that your XPA antibody (NB100-93321) detects?
A: Xeroderma pigmentosum group A-complementing protein (XPA) has an expected molecular weight of approximately 31 kD and this protein undergoes post-translational modifications (PTMs) such as phosphorylation as well as ubiquitination that leads to small increase in its observed molecular weight in Western blots. Our XPA antibody (NB100-93321) has been validated in whole cell lysate from HeLa cells, wherein it detected specific single band at 39kDa.
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