XPNPEP3 Antibody (OTI4B8)
Novus Biologicals | Catalog # NBP3-44641
![XPNPEP3 Antibody (OTI4B8) Immunohistochemistry;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-](https://resources.rndsystems.com/images/products/nbp3-44641_mouse-xpnpep3-mab-oti4b8-azide-and-bsa-free-immunohistochemistry-7520262247113.jpg "Immunohistochemistry;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-")
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI4B8
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Product Specifications
Immunogen
Full length human recombinant protein of human XPNPEP3(NP_071381) produced in HEK293 cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for XPNPEP3 Antibody (OTI4B8)
Immunohistochemistry;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XPNPEP3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![XPNPEP3 Antibody (OTI4B8) Immunocytochemistry/ Immunofluorescence;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-](https://resources.rndsystems.com/images/products/nbp3-44641_mouse-xpnpep3-mab-oti4b8-azide-and-bsa-free-immunocytochemistry-immunofluorescence-7520262259174.jpg "Immunocytochemistry/ Immunofluorescence;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-")
Immunocytochemistry/ Immunofluorescence;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-
Anti-XPNPEP3 mouse monoclonal antibody immunofluoreaining of COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP3.![XPNPEP3 Antibody (OTI4B8) Immunohistochemistry;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-](https://resources.rndsystems.com/images/products/nbp3-44638_mouse-pepd-mab-oti5d5-azide-and-bsa-free-immunohistochemistry-75202622435219.jpg "Immunohistochemistry;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-")
Immunohistochemistry;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XPNPEP3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![XPNPEP3 Antibody (OTI4B8) Flow Cytometry;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-](https://resources.rndsystems.com/images/products/nbp3-44641_mouse-xpnpep3-mab-oti4b8-azide-and-bsa-free-flow-cytometry-752026225581.jpg "Flow Cytometry;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-")
Flow Cytometry;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XPNPEP3 antibody, and then analyzed by flow cytometry.![XPNPEP3 Antibody (OTI4B8) Western Blot;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-](https://resources.rndsystems.com/images/products/nbp3-44641_mouse-xpnpep3-mab-oti4b8-azide-and-bsa-free-western-blot-75202622541717.jpg "Western Blot;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-")
Western Blot;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-XPNPEP3 monoclonal antibody.![XPNPEP3 Antibody (OTI4B8) Western Blot;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-](https://resources.rndsystems.com/images/products/nbp3-44641_mouse-xpnpep3-mab-oti4b8-azide-and-bsa-free-western-blot-752026231254.jpg "Western Blot;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-")
Western Blot;XPNPEP3 Antibody (OTI4B8)[NBP3-44641]-
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPNPEP3 (, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPNPEP3.Applications for XPNPEP3 Antibody (OTI4B8)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:500~2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
>95%
Reconstitution
For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized powder ( 1X PBS, pH 7.3,)
Preservative
8% Trehalose
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Calculators
Background: XPNPEP3
Alternate Names
Aminopeptidase P3, APP3probable Xaa-Pro aminopeptidase 3, NPHPL1EC 3.4.11.9, X-Pro aminopeptidase 3, X-prolyl aminopeptidase (aminopeptidase P) 3, putative
Gene Symbol
XPNPEP3
Additional XPNPEP3 Products
Product Documents for XPNPEP3 Antibody (OTI4B8)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for XPNPEP3 Antibody (OTI4B8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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